Study On Anti-tumor Mechanism Of Abnormal Savda Munziq In Vivo Based On Transplanted Cervical Cancer U27 Model

Objective:This study aims to investigate the antitumor effect of Abnormal Savda Munziq(ASMq) in vivo based on transplanted cervical cancer U27 model mice. Methods:Establishment of transplanted cervical cancer U27 model:168 mice were randomly divided in to 6 groups:control group,cyclophosphamide group, transplanted cervical cancer U27 modelgroup, ASMq low, medium, high dosage group(oral administration of ASMq respectively 2、4、8 g·kg-1·d-1 for 10 days). Indexes are measured in batch.(1) We observed mice tumor inhibition rate、 thymus index、 spleen index、 liver index and the index of kidny, variation of mouse serum IL-2, IFN-γ, TNF-α level, and by the MTT method detected the proliferation of spleen lymphocytes in mice and pathological conditions of tumor in mice.(2) Using Nuclear Magnetic Resonance Spectroscopy(1H-NMR) technology combined with pattern recognition and orthogonal partial least squares discriminant analysis(OPLS-DA) method analyze to seeking for the variability of serum metabolites and metabolic network pathways of transplanted cervical cancer U27 model mice`s after oral administration of different dose of ASMq and cyclophosphamide drug intervention.(3) The expression of TGF-β1 and TNF-α protein in transplanted cervical cancer U27 tumor tissue was detected by Western blot assay. Results:(1) The tumor inhibition rates of cyclophosphamide group and in the ASMq low、medium、high dosage group were 77.58%, 30.53%, 59.12% and 50.73% respectively, has obvious antitumor effect(P<0.05). Given ASMq later in serum IL-2、IFN-γ and TNF-α levels were significantly increased(P<0.05). ASMq can significantly promote the spleen tlymphocyte proliferation of transplanted cervical cancer U27 mice. Invasive growth and diffusion rate in tumor tissue were speed in the transplanted cervical cancer U27 model group,cell size and lists is disorder, had a cell mild edema. tumor tissue necrosis of tumor cells are smaller which in the ASMq medium, high dosage group.(2) Compared with control group, the concentration level of some metabolites were reduced in the transplanted cervical cancer U27 model group mice`s serum, including isoleucine, valine, alanine, Glutamine, glycine,amino acid and lactic acid, citric acid, creatine and 1-methyl histidine; while low density lipoprotein level was increased(P<0.05). Compared with the transplanted cervical cancer U27 model group, in the serum different doses of ASMq after oral administration for the transplanted cervical cancer U27 model group mice`s, the amino acids that isoleucine, leucine, valine, alanine, glutamine content was increased and glycoprotein, citric acid, creatine content also had a rising trend(P<0.05).(3) Compared with the transplanted cervical cancer U27 model group, the expression levels of TGF-β1 protein and TNF-α protein expression were significantly decreased in the mice tumor tissues in the cyclophosphamide administration group and different doses of ASMq administration group, and the difference was statistically significant(P<0.05). Conclusion:ASMq has some antitumor effects on transplanted cervical cancer U27 model mice in vivo, and it`s mechanism of treatment and prevention of tumor may be achieved not only by improving the immune function of transplanted cervical cancer U27 model mice, but also adjusting the disordered amino acid metabolism and energy metabolism, and by inhibiting the related protein expression levels of TGF-β1 protein and promoting the expression levels of protein TNF-α.