| Objective: To observe the therapeutic effect of platelet-derived growth factor receptor αRNA interference on inhibiting experimental proliferative vitreoretinopathy(PVR). Methods: Different concentrations of PDGFR-α shRNA were blended with lip 2000, and the final scale of PDGFR-αshRNA/lip2000 complex was 1:1 、 1:2and 1:3 respectively(the concentration of PDGFR-αshRNA was respectively 2μg、3μg and 4μg). All the complexes were cultivated in human retinal pigment epithelium for 24 hours after transfection and then respectively injected 0.1ml to rabbit vitreous cavity. 40 healthy adult rabbits were seleceted in this study and randomly divided into colored balanced salt solution(BSS) group(N), comprising group lipofectamineTM2000 HRPE cell dilution of(A group), The highest transduction efficiency of 1.0, 1.5 and 2.0μmol?L-1 containing PDGF-α receptors shRNA and lipofectamineTM2000 of HRPE cell dilution were selected(respectively group B, C and D) with 8 eyes each, the right eye as the experimental eye.The extent of PVR was observed by indirect ophthalmoscope; and slice staining situation was observed by immunohistochemistry. the fundus changes were observed by histopathology. Results: The highest transduction efficiency was PDGFR-αshRNA / lip2000 ratio of 1: 2; The extent of proliferative vitreoretinopathy, the histopathology changes and the immunohistochemistry of PDGFA in group B,C and group D were significantly lower than that in group A, while group D was more lower than group B and C. Conclusions: PDGF-α receptor RNA interference could inhibit the formation of experimental proliferative vitreoretinopathy.,and within a certain concentration range as the concentration increased inhibition enhanced. |
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