| Objective:(1) To detect the Polymorphism of the 10 SNPs of the APOA1 gene in Uyghur and Kazakh and explore the correlation between SNPs and dyslipidemia;(2) To analysis the haplotypes of the 10 SNPs of the APOA1 gene and dyslipidemia in Uyghur and Kazakh.Methods:(1) Randomized sampling 365(345) dyslipidemia patients as dyslipidemia group and370(391) non-dyslipidemia subjects for control group in Uygur(Kazak) from the data we collected before.(2) Snapshot methods was used to detect the APOA1 gene 10 SNPs.(3) All the calculations and statistics were performed with the computer program, SPSS version 20.0. Statistical analysis adopt χ2 test, t test and one-way logistic regression, and calculate the values of odds ratio and 95%confidence intervals for odds ratio. Linkage disequilibrium and haplotype were analysed by SHEsis software.Results:1. The 10 SNPs were conformed to Hardy-Weinberg equilibrium in Uyghur and Kazakh(all P >0.05).2. In Uyghur, the frequency of APOA1 gene rs670 genotype and allele were different in dyslipidemia group and control group(χ2 = 5.891, P = 0.048; χ 2= 6.217, P = 0.013), no significant difference for the other 5 SNPs(all P > 0.05). In Kazakh, no significant differences in frequency distributions of three genotypes and alleles of the 10 SNPs were noted between the dyslipidemia group and the control group(P > 0.05).3. In Uyghur, carry with rs5072(rs2070665) CT and CT/TT genotypes were 1.388(1.398) and1.346(1.346) times than those with CC genotype to get dyslipidemia; carry with rs670 GA and GA/AA genotypes were 1.441 and 1.482 times than those with GG genotype to get dyslipidemia,compared with G allele, carrying A allele were 1.436 times more likely to get dyslipidemia. After gender stratification in male Uyghur, carry with rs7116797 GA and GA/AA genotypes were 2.023 and 1.784 times than those with GG genotype to get dyslipidemia; carry with rs5072 and rs2070665 CT and CT/TT genotypes were all 1.915 and 1.829 times than those with CC genotype to get dyslipidemia, compared with C allele, carrying T allele were all 1.481 times more likely to get dyslipidemia; carry with rs670 GA and GA/AA genotypes were 1.840 and 1.697 times than those with GG genotype to get dyslipidemia.4. The levels of TG in GG genotype in Uygur dyslipidemia group of rs670 was significantly lower than those in GA/AA genotype(P < 0.05); in female Kazakh, the levels of TG、LDL-C and APo B in GG genotype of rs12718464 were higher than those in GA/AA genotype, the levels of TG in GG genotype in dyslipidemia group of rs670 was higher than those in GA/AA genotype as well as the levels of HDL-C in control group(all P < 0.05); in male Kazakh, carry with rs5069 CC genotype in dyslipidemia group has a higher level of HDL-C than those with CT/TT genotype, yet carrying rs632153 GG genotype has a lower level of HDL-C compared with GT/TT genotype.5. In Uyghur, Significant LD were observed between rs5072 and rs7116797, rs5072 and2070665, rs2070665 and rs7116797, rs5069 and rs5076; perfect LD was observed between rs12718464 and rs12721026. In Kazakh, Significant LD were observed between rs5072 and rs7116797, rs5072 and 2070665, rs12718464 and rs12721026, rs2070665 and rs7116797, rs5069 and rs5076; perfect LD was observed between rs5072 and rs2070665.6. In Uyghur,11 haplotypes were found between APOA1 gene 8 loci(rs12721026, rs5076,rs7116797, rs12718464, rs5072, rs2070665, rs5069, rs670) and the frequency of TCAGTTCG and TCGGCCCA haplotype in the dyslipidemia group was higher than that in the control group(all P< 0.05),the other haplotypes distribution were not significantly different between two groups. In Kazakh, 10 haplotypes were found between APOA1 gene 8 loci(rs12721026, rs5076, rs7116797,rs12718464, rs5072, rs2070665, rs5069, rs670) and no significant differences were found of haplotypes between dyslipidemia group and control group(all P > 0.05).Conclusions:(1) APOA1 gene rs670 Polymorphism may be related to dyslipidemia in Uyghur, no correlation were found of other 9 SNPs and dyslipidemia. In 10 SNPs of APOA1 gene may not associated with dyslipidemia in Kazakh.(2) CT and CT/TT genotypes of rs5072 and rs2070665,GA and GA/AA genotypes and A allele of rs670 were maybe risk factors for dyslipidemia in Uyghur. After gender stratification, CT and CT/TT genotypes and T allele of rs5072 and rs2070665, GA and GA/AA genotypes of rs670 and rs7116797 were may be risk factors for dyslipidemia in male Uyghur.(3) The A allele of rs670 may leaded to lower levels of TG and LDL-C in Kazakh and higher level of TG in Uyghur. The T allele of rs5069 may leaded to lower level of HDL-C in Uyghur. The A allele of rs12718464 may leaded to lower TG and LDL-C in female Uyghur.(4) In Uyghur, Significant LD were observed between rs5072 and rs7116797,rs5072 and 2070665, rs2070665 and rs7116797, rs5069 and rs5076; perfect LD was observed between rs12718464 and rs12721026. In Kazakh, Significant LD were observed between rs5072 and rs7116797, rs5072 and 2070665, rs12718464 and rs12721026, rs2070665 and rs7116797,rs5069 and rs5076; perfect LD was observed between rs5072 and rs2070665.(5) The TCAGTTCG and TCGGCCCA haplotypes may serve as risk factors of dyslipidemia in Uyghur. |
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