Studies On The Chemical Constituents Of Sedum Sarmentosum Bunge

Sedum sarmentosum Bunge is the fresh or dry whole plant of Sedum sarmentosum Bunge of Sedum L.plant of Crassulaceae.There are about 470 species of Sedum L,including 124 species,1 subspecies,14 variants and 1 variant in China.Sedum sarmentosum Bunge can affect the liver,gallbladder,small intestine.It has the efficiency of detoxification and it can be used for the treatment of jaundice,urinary adverse,carbuncle,sore and acute and chronic hepatitis.Researchers had found that Sedum sarmentosum Bunge has pharmacological activity of anti-tumor,anti-oxidation,enhancing muscle strength and so on.Objective: The High performance liquid chromatography can be used to expore the most efficient extraction of solvents which can be used to extract the whole dried plant of Sedum sarmentosum Bunge and to get the monomer compounds from the crude extraction and to explore the function of five kinds of monomer compounds for protecting the liver and to establish a high performance liquid chromatography as a means of content determination method.Methods: The High performance liquid chromatography were used to expore the most efficient extraction of solvents and the fingerprints of ethanol extract of 70% ethanol were explored.The reflux extraction method can be used to extract Sedum Sarmentosum Burge and then get the extracts.The extracts were separated by silica gel column chromatography,Sephadex LH-20 gel column chromatography,carbon XIII bonded silica gel reversed column chromatography,TLC preparative chromatography and preparative high performance liquid chromatography and refined by recrystallization to obtain monomer compounds.The structures of monomer compounds were identified by its physical and chemical properties,LC-MS and NMR.Injury model of liver in vitro was established and the protecting impacts of liver of the five monomer compounds was explored;HPLC was used to determine the content of ?-amyrione.Results: The most compounds were extracted from 70% ethanol extract and the extract efficiency was the highest.Ninteen kinds of monomer compounds were isolated from 70% ethanol extract,as following: ?-amyrione(I),?-amyrin(II),3-epi-?-amyrin(III),?-sitosterol(IV),luteolin(V),?-amyrin(VI),oleanolic acid(VII),kaempferide(VIII),isorhmnetin(IX),hyperoside(X),tricin-7-O-?-D-glucoside(XI),apigenin(XII),vanillic acid(XIII),quercitrin(XIV),daucosterol(XV),quercetin(XVI),stigmasterol(XVII),gallic acid(XVIII),luteolin-7-O-?-D-glucoside(XIX).Compounds XII and XIII were isolated from this genus for the first time,compounds VI,VII XVII,XVIII were isolated from Sedum sarmentosum Bunge for the first time.The results showed that all the five compounds had hepatoprotective activity and the activity of compound X was the strongest.A method for the content of ?-amyrione in Sedum sarmentosum Bunge was established by high performance liquid chromatography,showing that this method is simple and had high specificity,high precision,high reproducibility and low interference.Conclusions: The most compounds were extracted from 70% ethanol extract and the extract efficiency was the highest.Ninteen kinds of monomer compounds were separated and identified from Sedum sarmentosum Bunge and compounds XII and XIII were isolated from this genus for the first time and compounds VI,VII XVII,XVIII were isolated from Sedum sarmentosum Bunge for the first time.Compounds I?II?VI?X?XII both had hepatoprotective activity and compound X had the strongest hepatoprotective activity.A high performance liquid chromatography method for the content of ?-amyrione was established,the method is simple,rapid and accurate.It provides a powerful method for the quality evaluation of the medicinal materials of Sedum sarmentosum Bunge.The method is simple and had high specificity,high precision,high reproducibility and low interference.