Study On The Chemical Constituents And Antitumor Activity From The Heartwood Of Trachycarpus Fortunei(Hook.) H.Wendl.

Tumor is the second largest killer to the cardiovascular disease which is one of the hazards to human health disease.Chemotherapy be used to cure tumor in clinical will cause huger damage of normal tissue to the human body and cause a higher risk of adverse reactions.It is an effective way that found the active compounds from natural products and developed less side effects of antineoplastic drugs.Trachycarpus fortunei(Hook.)H.is the plant of the Trachycarpus family.According to previous research,we have found that the extract from the heartwood of Trachycarpus fortunei show antitumor activity in vitro for human breast cancei cell line(MCF-7)?human gastric carcinoma cell line(BGC-823).In ongoing research on antitumor constituents rom this plant,the ethanol extract of the plant were subjected to chromatographic purification and isolation,twenty two compounds were isolated.Fifteen of them were determined by physicochemical properties and spectral date,and elucidated as 3,5-dihydroxy-2-methyl-?-pyranone(1),tetracosanic acid(2),pyrocatechol(3),?-droxyanthraquinone(4),?-sitosterol(5),4-hydroxy-2,6-dimethoxy-benzoic acid(6),3,5-dihydroxy-?-pyranone(7),5-hydroxy-2-hydroxymethyl-?-pyranone(8),4-hydroxybenzoic acid(9),daucosterol(10),mollugin(11),1,3-dihydroxyanthraquinone(12),2-methyl-1,3,6-trihydroxy-9,10-anthraquinone-3-O-?-rhamnosyl(1?2)-?-D-glucoside(13),Hypoglaucin H(14),Dioscin(15).There are six compounds' structure was being identified.Compounds 1?2?4?6~8?10~14 were obtained from this plant for the first time,and 1?2?4?6~8?11~13 were obtained from the genus for the first time.Finally,the compounds were examined for the antitumor and anti-oxidative activity in vitro.The screening results of antitumor activity in vitro show that the quinone compounds had good antitumor activity and the half inhibitory concentration IC50 of compound 11 for human osteosarcoma cell line(U20S)were 18.1?g/m L.The antioxidant activities of compounds were determined in vitro by scavenging OH and ABTS radical assay.Finally,compound 11 exhibited higher radical-scavenging capacities than other compounds which were involved in the experiment.