Gene Delivery Of IL-33 Through BMSCs Influence The Inflammation And Cardiac Function Of Myocardial Infarction

Objective:By increasing the expression level of IL-33 in rat bone marrow mesenchymal stem cells(BMSCs),the modified BMSCs was transplanted into the heart of rats with myocardial infarction,and the effects of these changes on myocardial inflammation and its function after myocardial infarction were observed.Methods:Isolated and cultured of BMSCs from the bone of SD rat,construction of the high expression of IL-33 lentivirus,and the BMSCs infection,the expression level of IL-33 in the regulation of BMSCs,BMSCs and IL-33 were collected after impact on the activity of BMSCs,apoptosis.BMSCs infected with lentivirus co-cultured with T cells and mono-macrophage cells to detect the inflammation and cardiac function.Rat myocardial infarction model was created by ligating the left anterior descending coronary artery.Then different BMSCs(PBS,BMSCs-Vector,BMSCs-IL-33)were injected through intramyocardial injection,and then detect the inflammatory level and cardiac function after5 or 14 days.Results:BMSCs apoptosis reduced after pCDH-IL-33 transfection.BMSCs-IL33reduce the proliferation of T cell of spleen and influence the polarization of macrophage to CD206~+macrophage.We also found transplantation of BMSC-IL-33 reduced inflammation and enhanced cardiac function of rats with myocardial infarction.Conclusion:Above all,our study successfully established the IL-33-overexpressed BMSCs.We found BMSCs-IL-33 can obviously reduce myocardial infarction area and improve cardiac function after myocardial infarction.This phenomenon may be caused by the decrease of BMSCs'apoptosis and inhibition T cell proliferation,and influence the polarization of mono-macrophages.Thus,our study provided a theoretical basis for the further applyment of gene-modified BMSCs for the treatment of relevant diseases.