Effects Of Tang Shen Kang On Expression Of TGF-?1 And VEGF In Renal Tissue Of Diabetic Rats

Objective: To study the effect of the treatment of Tang Shen Kang on the expression of TGF-1 and VEGF in kidney of diabetic rats.Methods: 1.To establish rats model of diabetic nephropathy:select healthy male SD rats,fed with high glucose and high fat diet + unilateral nephrectomy + intraperitoneal injection of streptozotocin(STZ)models.Mold standard: non fasting blood glucose,16.7mmol / L,24 hours urine protein quantitative 30 mg.2.Group:SD rats were randomly divided into model group(model group),perindopril group(western medicine group),Tang shen kang low dose group(low dose group),Tangshenkang high dose group(high dose group),and normal SD rats as control group(control group).3.Medication:After the models established successfully,inject them1 times daily,2ml each time,the concentration of high dose group(16g/kg/d),low dose group concentration(8g/kg/d)),western medicine group was given by gavage 15mg/kg/d,saline control group and model group were given intragastric administration intervention,8 weeks.4.Evaluation method:After the intervention,measured the weight,24 h urinary protein and fasting blood glucos of rats in each group;take abdominal aortic blood urea nitrogen after anesthesia(BUN),serum creatinine(Scr),triglyceride,cholesterol,kidney,pathologic examination and weighing of renal tissue.5.Organize regular sections parallel hematoxylin and eosin(HE)staining to observe the renal pathological changes under light microscope;the expression was detected by immunohisto chemistry in renal tissue of rats with diabetic nephropathy TGF-?1 and VEGF proteins,using SPSS17.0 statistical analysis software for statistical processing of experimental data,P < 0.05,with statistical significance,P < 0.01,there was statistical significance.Result 1.Blood glucose:the blood glucose levels of the model group and the treatment group after treatment were significantly higher(P<0.01),and the low dose group(P<0.05);24h urine protein:before drug intervention;in the model group,high and low dose group and western medicine group urine protein were significantly increased(P<0.01),but there was no difference between the groups(P > 0.05).Drug intervention;compared with the model group,the treatment group and the control group,24 h urinary protein were significantly increased(P<0.01),high and low dose group compared with the western medicine group significantly lower 24 h urinary protein(P<0.01),but the high,low dose group showed no significant difference(P > 0.05);Serum creatinine:the model group,high and low dose group and western medicine group compared with the control group,the serum creatinine increased(P<0.05),compared with the model group,high and low dose group,western medicine group were significantly lower serum creatinine(P<0.01);Urea nitrogen:model group,high and low dose group and Western medicine group urea nitrogen significantly increased(P<0.01),compared with the model group,the high and low dose group and western medicine group urea nitrogen decreased(P<0.05).2.Pathological changes:under light microscope,the structure of glomerular formation in rats in control group was observed.In the model group,the proliferation of glomerular mesangial matrix was diffuse,the glomerulus was partially adherent,the renal tubular epithelial cells showed vacuolar degeneration,inflammatory infiltration and fibrosis were observed in the interstitium.Glomerular lesions in each treatment group were significantly lower than those in the model group.3.The control has a small amount of expression of TGF-? 1 and VEGF group of glomerulus and renal tubules in rats;the expression of TGF-?1 and VEGF increased compared with the control group,mainly in the glomerular mesangial area,but renal interstitial and renal tubular epithelial cells also have basal expression.Compared with the model group,the expression of TGF-?1 and VEGF in the treatment group decreased significantly,especially in the low dose group,but was still higher than that in the control group.Conclusion:(1)Tang Shen Kang can effectively reduce the urinary protein,creatinine and urea nitrogen of DN rats,and effectively reduce the pathological changes of renal tissue in DN rats.(2)It can protect the kidney by inhibiting the expression of TGF-beta 1 and VEGF protein,thus delaying the progression of DN.