Study About The Expression Of CypA-ERK1/2 Pathway And Its Blocking Effect In The Hippocampus From FMR1 KO Mice By Electroacupuncture Of Changqiang(GV1) Acupoint

Objective:This paper aims to discuss the effect of eletroacupuncture at Changqiang(GV1)acupoint on the expression of FMR1 gene knockout mice in the hippocampus region of CypA、ERK1/2 and CREB proteins,and to enrich the understanding of the relationship between the Changqiang(GV1)acupoint of electroacupuncture and the improvement of learning and memory ability.Methods:Identification of murine gene phenotype by polymerase chain reaction,and choosing a Fragile X-1 deficiency in the KO mice with the age of 25th,randomly divided into blank group,Changqiang(GV1)acupoint group,non-acupoints group and blocking agent Changqiang(GV1)acupoint group,with 6 mice in each group,total 24 mice.All groups of mice were treated with daily fixed time,once a day,continuous intervention for 14 days.Blank group was given grab action,the rest of the group to take electric acupuncture intervention by HANS eletroacupuncture therapeutic apparatus.The group of eletroacupuncture chooses the 30th number 1 inch acupuncture to make the double pole needle to puncture,the puncture depth achieves 10mm were connected with continuewave,2Hz,2mA,30min.The experimental mice were tested by autonomic activity during the two days before and after the intervention treatment.All groups of mice were cr.aniotomy for brain at 43 days of age,and the expression of the related proteins of CypA,ERK1/2 and CREB was detected by Western Blot and immunohistochemistry.Analyses the data by means of one-way analysis of variance,and the paired T test was used to analyze the independent activity standing before and after intervention.Results:(1)The independent activity experiment revealed,’there were no significant changes before the intervention treatment(P>0.05).The independent activity behavior of the Changqiang(GV1)acupoint group was significantly lower than that of blank group and non-acupoints group after the intervention treatment(P<0.05).The independent standing behavior of the Changqiang(GV1)acupoint group and blocking agent Changqiang(GV1)acupoint group was significantly lower than that of non-acupoints group after the intervention treatment(P<0.05).The independent standing and activity behavior of the Changqiang(GV1)mice after intervention were significantly lower than that before intervention(P<0.05).(2)The expression of CypA、CREB by immunohistochemistry in the Changqiang(GV1)acupoint group was significantly higher than that of blocking agent Changqiang(GV1)acupoint group、non-acupoint group and blank group(P<0.05).The expression of ERK1/2 by immunohistochemistry in the Changqiang(GV1)acupoint group was significantly higher than that of blank group and blocking agent Changqiang(GV1)acupoint group(P<0.05);The expression of ERK1/2 by immunohistochemistry in the non-acupoint group was significantly higher than that of blank group(P<0.05).(3)The expression of CypA by Western Blot in the Changqiang(GVl)acupoint group was higher than that of blocking agent Changqiang(GV1)acupoint group、non-acupoint group and blank group(P>0.05).The expression of CypA by Western Blot in the non-acupoint group and blank group was higher than that of blocking agent Changqiang(GV1)acupoint group(P<0.05).The expression of ERK1/2、CREB by Western Blot in the Changqiang(GV1)acupoint group was higher than that of blocking agent Changqiang(GV1)acupoint group、non-acupoint group and blank group(P<0.05).Conclusion(1)Acupuncture Changqiang(GV1)acupoint can adjust the independent activity of FMR1 gene knockout mice.(2)Acupuncture Changqiang(GV1)acupoint can promote the expression of CypA、ERK1/2、ERB proteins in the hippocampus region of FMR1 gene knockout mice;the blocker PD98059 can inhibit the expression of CypA、ERK1/2、CERB proteins.