The Experimental Study On The Regulatory Effect Of MicroRNA-7 On Intestinal Mucosal Protective Factor TFF3 In Rats With Inflammatory Bowel Disease

Objective: To investigate the expression and correlation of mi R-7 and TFF3 in TNBS-induced IBD animal model,and try to clarify the regulating effects of miR – 7 on intestinal mucosal protection factor of TFF3.Methods:Sixty male SD rats(3-4 weeks old)were were randomly divided into 3 groups,TNBS group,Normal saline group and Ethanol group.Young rats were givern TNBS to establish animal model of inflammatory bowel disease.Body weight,and disease activity index score were observed every day,to observe intestinal inflammatory change and colon tissue injury score by HE staining to confirm colitis exist.Detecting the gene expression of mi R-7 and TFF3 by Real-time RT-PCR,and the expression of TFF3 protein by immunohistochemical method to explore the the differences of mi R-7 and TFF3 in colonic tissues.Forty male Balb/c rats(3-4 weeks old)were were randomly divided into 4 groups,TNBS group,Normal saline group,miRNA-antagomir group and recombinant TFF3 groups.The colon tissue damage degree,gene expression miR-7 and TFF3 by PCR,and protein expression of TFF3 in intestinal tissue by western blot were used to evaluate regulation function of miR-7 on TFF3.Results:The body weight is obviously decreased after TNBS,while disease activity index and pathological damage score increased significantly.body weight drop loss,DAI score and pathological score are gradually returned to normal with the change of time,compared with NS group the difference was statistically significant in colitis rats,miR-7 and TFF3 gene levels wre significantly uptegulated and downregulated,respectively,and inversely correlated.miR-7 antagomir administration potently suppressed miR-7 expression,increased expression of TFF3 gene by Real-time RT-PCR and increased TFF3 protein expression by IHC and Western Blot in the mouse colon,compared with NS group was statistically difference.Conclusion: miR-7 and TFF3 gene levels wre significantly uptegulated and downregulated,respectively,and inversely correlated.mi R-7 antagomir administration potently suppressed mi R-7 expression and increased expression of TFF3 gene and protein in the mouse colon.