| Objective:In this study,the model of focal cerebral ischemia reperfusion in rats was taken as the research object,and the method of exercise therapy in water was taken as an intervention method,with random movement obstacle,nerve function score,muscle tension,histological examination,gamma aminobutyric acid(GABA)and glutamic acid(Glu)content as evaluation criteria,to observe the exercise therapy in water.The effects of the inhibitory neurotransmitters and excitatory neurotransmitters on the expression of excitatory neurotransmitters in the treatment of cerebral hemiplegic spasm after ischemic stroke are explored to provide a theoretical basis for the clinical application of water exercise therapy and to provide a reference for the further study of water exercise therapy.Methods:A total of 60 240-270 g SD rats were divided into three groups according to the MCAO model group: sham-operation group and blank group.The ratio of animals to 4:1:1 was randomly divided into three groups.After grouping,there were 20 animals in the treatment group and the model group,and 10 animals in the sham-operation group and the blank group.MCAO model group rats were randomly divided into two groups: model group and treatment group.The middle cerebral artery occlusion(MACO)model was established in rats in the model group and treatment group.The sham-operation group only separated the right common carotid,external and internal carotid arteries,while the blank group did not do any treatment.After establishing model for 3days,the treatment group was treated with exercise therapy in water,30 min a day,lasting 6 days.The blank group,sham-operation group and model group were free to exercise,and no special treatment was given.The random movement obstacle score,nerve function score and muscle tone score were performed before and after treatment,respectively.At the end of the treatment,the rats were killed and the right brain tissue was removed on the super clean surface.The pathological changes of the right cerebral cortex in each group were observed under light microscope.The expression of GABA and Glu in each group was measured by ELISA.Results:1.Neurological score and voluntary motor function score results: before treatment,compared with control group and sham-operation group,the difference was not statistically significant(P > 0.05);compared with the blank group and model rats,the difference was statistically significant(P<0.05),the model group and the treatment groups had no statistically significant difference after treatment;compared with the model group,the treatment group scores were significantly lower((P<0.05);2.Muscle tone assessment: electrophysiography was used to assess muscle tone.Before treatment,compared with blank group and sham-operation group,the difference was not statistically significant(P>0.05);compared with the blank group,model group and treatment group increased muscle tone,the difference was statistically significant(P<0.05),but there was no significant difference between these groups(P>0.05);after modeling after treatment,compared with the model group,the muscle tone in treatment group improved,the difference was statistically significant(P<0.05)3.Pathological HE slices showed:(1)in model group,brain tissue was extensively denatured,the nucleus was mostly condensed,cytoplasmic edema and hemorrhage,inflammatory cells infiltration,blood cells leakage in some areas and microglia hyperplasia.(2)In the treatment group,there was still a wide range of liquefaction necrosis in the brain tissue,the nuclear condensation,the disappearance of nucleolus,the swelling of the cytoplasm,and the obvious increase of microglia under the microscope.(3)There was no obvious abnormality in the nerve tissue of the shamoperation group and the blank group.4.ELISA results showed :for the expression of GABA,compared with blank group and sham-operation group,the difference was not statistically significant(P>0.05);compared with the blank group,increased expression of GABA in brain tissue of rats in the model group and treatment group were statistically significant(P<0.05);compared with the model group,the expression of GABA in brain tissue of treatment group rats increased significantly(P<0.05).Glu expression: the difference between the sham-operation group and the blank group was not statistically significant(P>0.05).Compared with the blank group,the expression of Glu in the brain tissue of the model group increased(P<0.05),and the increased amplitude of the rats in the treatment group was not statistically significant(P>0.05).Compared with the model group,the expression of Glu in the brain tissue of the treatment group was low(P<0.05).Conclusion:1.Aquatic exercise therapy can relieve the limb spasm in ischemic stroke rats and improve the motor function of the hemiplegic rats.2.Aquatic exercise therapy can accelerate the repair of necrotic brain tissue,and promote the recovery of neural function in rats.The increase of microglia in the brain tissue of rats and its neuroprotective effect may be one of its mechanisms.3.Aquatic exercise therapy can reduce the expression level of Glu in the central nervous system.Glu may play an important role in the treatment of hemiplegic limb spasticity.4.The GABA expression level of high altitude pith in aquatic exercise therapy,and the increase of GABA inhibitory effect may be one of the mechanisms of water exercise therapy to improve hemiplegia limb spasm. |
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