Preparation Of Extract From Leaves Of Cyclocarya Paliurus(batal.)iljinsk.Leaves

Objective:Cyclocarya paliurus?Batal.?Iljinsk.leaves are often used for drinking tea,which has the effect of treating diabetes mellitus.In this paper,the extract of Cyclocarya paliurus?Batal.?Iljinsk.leaves was prepared by using the Cyclocarya paliurus?Batal.?Iljinsk.leaves raw material.The preparation process,quality standard?draft?,preliminary stability,preliminary pharmacodynamics,etc.were studied to provide the basis for the development of hypoglycemic preparations in the future.Methods:?1?Study on extraction technology:Used the method of water frying and boiling.Used the method of single factor investigation and L₉?3⁴?orthogonal test.The optimum extraction technology of the extract of Cyclocarya paliurus?Batal.?Iljinsk.leaves was selected by taking the content of isocercetin and the percentage of dry paste as the index,the amount of water adding,the extraction time and the times of extraction as the investigation factors.?2?Molding technology research:Freeze drying method,through single factor investigation method,With vacuum degree,drying time and drying temperature as the investigation factors,the molding technology of the extract of Cyclocarya paliurus?Batal.?Iljinsk.leaves was optimized.?3?Study on the quality standard:According to the General Principles of Preparation of Chinese Pharmacopoeia?2015 edition?,thequality standard of the extract of Cyclocarya paliurus?Batal.?Iljinsk.leaves officinalis?draft?and the extract from the leaves of Cyclocarya paliurus?Batal.?Iljinsk.leaves were established,and to determine the content of Cyclocarya paliurus?Batal.?Iljinsk.leaves instant powder in Isoquercitrin,making the content limit;according to the national standard of food hygiene,the quality standard?draft?of the extract of edible Cyclocarya paliurus?Batal.?Iljinsk.leaves was established,and the requirements of sensory,physical and chemical indexes and microorganism were formulated.?4?Study on the stability:According to the Principles of Chinese Pharmacopoeia?2015 edition?,carried out an experiment on the influencing factors of the extract of Cyclocarya paliurus?Batal.?Iljinsk.leaves,and accelerated and long-term tests to investigate the stability.?5?Preliminary Pharmacodynamics study on hypoglycemic effect:The model of type 2 diabetes induced by alloxan in mice was used to investigate the effect of the extract of Cyclocarya paliurus?Batal.?Iljinsk.leaves on hypoglycemia in type 2 diabetic mice.Results:?1?The extraction technology of the extract of Cyclocarya paliurus?Batal.?Iljinsk.leaves was determined.The best extraction process was:extract 2 times,and add 20 times water to each time.Extract30min and extract 1h.?2?The forming technology of the extract of Cyclocarya paliurus?Batal.?Iljinsk.leaves was determined.The optimum molding technology was as follows:the concentrated liquid of Cyclocarya paliurus?Batal.?Iljinsk.leaves with a solid content of 4.2 was taken for freeze-drying,vacuum of 90Pa in drying room and drying time of 48h.Drying temperature is-45?,dry powder,packaging,that is.?3?The quality standard?draft?of the extract of Cyclocarya paliurus?Batal.?Iljinsk.leaves.Qualitative Identification of Isoquercetin in preparation by TLC,the results of chromatographic spots were clear;HPLC method for quantitative analysis of Isoquercitrin method is reliable,stable and feasible;the properties,granularity,appearance evenness,moisture content,difference of loading quantity and content of instant powder were investigated,the results comply with the relevant regulations.?4?The stability of the instant powder was good.The results of influence factor test,accelerated test and long-term test show,There were no significant changes in characters,identification,examination and content of the extract of Cyclocarya paliurus?Batal.?Iljinsk.leaves,which were in accordance with relevant regulations.?5?The instant dissolving powder of Cyclocarya paliurus?Batal.?Iljinsk.leaves has better hypoglycemic effect.The low,middle and high dose of Cyclocarya paliurus?Batal.?Iljinsk.leaves instant powder could significantly reduce the fasting blood glucose,triglyceride,low density lipoprotein cholesterol of mice,and the high and middle doses were significant,the difference was statistically significant?P<0.05 or P<0.01?.Conclusion:?1?The preparation process of the extract of Cyclocarya paliurus?Batal.?Iljinsk.leaves was safe and reliable,and the reproducibility was good.?2?The quality standard?draft?of the extract of Cyclocarya paliurus?Batal.?Iljinsk.leaves is feasible,the quality is controllable,and the qualitative and quantitative analysis of the separation degree is good,the sensitivity is high,the reproducibility is good,and the inspection conforms to the relevant regulations.?3?Preliminary stability of the extract of Cyclocarya paliurus?Batal.?Iljinsk.leaves is good,which provides a theoretical basis for the preparation process and the feasibility of packaging materials.?4?The extract of Cyclocarya paliurus?Batal.?Iljinsk.leaves has a hypoglycemic effect on type 2 diabetic mice.