Effects Of Lingguizhugan Decoction On Heart Function And Ventricular Remodeling Of Chronic Heart Failure Rate

1.PurposeUsing a adriamycin-induced rat model of chronic heart failure,we seek to observe the efficacy of Linggui Zhugan Decoction(LZD)for chronic heart failure,and the effect of LZD on BNP,AngⅡ and ET-1 was also determined to investigate the mechanism of its efficacy,so as to provide scientific basis for further clinical application of this decoction.2.Method After adaption for 1 week,40 SD rats were randomly divided into a model group that received intraperitoneal injection of adriamycin(1.5 mg/kg,2 times a week for 4 weeks)to induce chronic heart failure,and a control group were injected with equal volume of normal saline in place of adriamycin.Two weeks after the last injection,successfulness of model construction was verified by heart echocardiography and pathological examination.Rats of the model group were further divided into an untreated group,a digoxin group and a LZD group that were given the specified treatment.Digoxin was administered at 0.026mg/kg/d,and LZD at 6.83g/kg/d,both by garage feeding in 1ml/100 g drinking water for 4 weeks,and the control and the untreated group were given equal volume of water.The doses were adjusted every day according to weight of the rats.At the end of treatment,echocardiography and pathological examination were performed for each group of rats,and BNP,AngⅡI,ET-1 levels were determined.3.Results1)General condition: compared to the control group,the untreated group showed significant manifestations of chronic heart failure,while the treated groups also showed poorer general conditions,but the symptoms of heart failure were milder and delayed.2)Myocardial morphological changes: compared to the control group,the untreated group showed loosely arranged muscle fibers with fibroblast proliferation and focal degeneration,while myocardial morphology of the treated groups was somewhat improved.3)BNP,ET-1,and Ang Ⅱ levels: these factors were significantly elevated in the untreated group compared to the control group(P<0.05),and such elevation was significantly reversed in the treated groups(P<0.05);Ang Ⅱ levels of the LZD group and the digoxin group were comparable(P>0.05),while the decreases of BNP and ET-1 in the LZD group and the digoxin group were comparable(P<0.05).4.Conclusion1)LZD effectively improved the symptoms of chronic heart failure and reduced mortality of the rats.2)LZD effectively improved heart function3)LZD delayed myocardial morphological changes in rats with chronic heart failure.4)LZD reversed the elevation of BNP,ET-1,and Ang Ⅱ levels in the rat model of chronic heart failure,inhibited excessive activation of the RASS system,and therefore inhibited myocardial remodeling and delayed progression of heart failure.