Effect Of Moxibustion At Shenque (CV8) On Monoamine Neurotransmitters And Free Radical Metabolism In Rat’s Hippocampus After Different Degrees Of Exhaustive Excise

Objective: To investigate the effect of moxibustion at Shenque(CV 8)on monoamine neurotransmitters and free radical metabolism in rat’s hippocampus after different degrees of exhaustive exercise.To explore the differences in the effects of different degrees of exercise central fatigue recovery and its possible mechanism.Methods: A total of 72 male Sprague-Dawley(SD)rats were randomly divided into a normal group(n=8),an untreated group(n=32)and a moxibustion group(n=32).According to the times of modeling or treatment,the untreated group was further randomly and equally divided into 1st,4th 7th and10 th trails subgroup(n=8);the moxibustion group was also further randomly and equally divided into 1st,4th,7th and10 th trails subgroup(n=8);Rats in both untreated and moxibustion subgroups were subjected to establishing the swimming exhaustive model.Rats in each moxibustion subgroup received mild moxibustion at Shenque(CV 8)for 15 min immediately after modeling,once every other day.The levels of monoamine neurotransmitters 5-hydroxytryptamine(5-HT),Dopamine(DA),Norepinephrine(NE)And malic dialdehyde(MDA),as well as the activities of superoxide dismutase(SOD),glutathione peroxidase(GSH-Px)and total antioxidant capacity(T-AOC)in rat’s hippocampus in each group were detected 24 h after the exhaustive exercise.Results: Compared with the 1st untreated subgroup,the exhaustive swimming time of rats was significantly prolonged in the 4th untreated subgroup(P<0.01)while it was significantly shortened in the 7th and 10 th untreated subgroups(all P<0.01),compared with the matched untreated subgroup,the exhaustive swimming time of rats in the 7th and10 th moxibustion subgroups was significantly prolonged(both P<0.01),Compared with the normal group,the 5HT、DA、NE and MDA concentration was increased significantly(all P<0.01)and the activities stress response of SOD and T-AOC were increased in the 1th untreated subgroup(both P<0.05);the 5-HT、DA、NE and MDA concentration was increased(all P<0.01),and the activities of SOD,GSH-Px and T-AOC were decreased differently(P<0.05 or P<0.01)in the 4th untreated subgroups.The 5-HT、NE、and MDA concentration was increased(P<0.05 or P<0.01),and the DA concentration and the activities of SOD,GSH-Px and T-AOC were decreased differently(P<0.05 or P<0.01)in the 7th untreated subgroups.The 5-HT、and MDA concentration was increased(both P<0.01),and the DA concentration and the activities of SOD,GSH-Px and T-AOC were decreased differently(all P<0.01)in the 10 th untreated subgroups.Compared with the matched untreated subgroup,the concentration of MDA was significantly reduced(P<0.05),the concentration of DA、NE and the activities of SOD、GSH-Px and T-AOC were significantly increased in the 4th moxibustion subgroups(P<0.05 or P<0.01);the concentration of MDA and 5-HT was significantly reduced(p<0.01).the concentration of DA、NE and the activities of SOD,GSH-Px and T-AOC were significantly increased in the 7th and10 th moxibustion subgroups(all P<0.01)Conclusion:Moxibustion at Shenque(CV8)can effectively prolong swimming time in rats with different degrees of repeated exhaustion.regulate the content of monoamine neurotransmitters in rat hippocampus,promote the recovery of nerve cells,and improve the ability of antioxidant and free radical metabolism,In this way,the anti-central fatigue effect can be brought into play.However,there are some differences,Among them,the regulation effect of moxibustion 7th and 10 th is the most obvious.