Association Of PNPLA3 Gene Polymorphism And Serum Triglyceride With Fatty Liver Disease

Objective: To explore the association of PNPLA3 rs738409 gene polymorphism and serum triglyceride(TG)levels with fatty liver disease(FLD)in Chinese Han people,and explore whether there is an interaction between them affecting the risk of FLD.Methods: Case control study was adopted.The study population was physical examination population from Ezhou City Center for Disease Control and Prevention in Hubei province.Abdominal ultrasound was used to screen FLD patients,and gender and age(3 years old)were used for 1:1 matching in the control group,a total of 886 subjects were included,including 443 in the FLD group and 443 in the control group.Basic information of subjects was collected through questionnaire survey and physical examination,blood samples were collected,basic biochemical indexes were detected by automatic biochemical analyzer,and DNA was extracted,PNPLA3 rs738409 gene polymorphism was detected by Mass Array system.Conditional Logistic regression analysis was used to evaluate the correlation between serum TG levels,PNPLA3 rs738409 gene polymorphism and FLD risk respectively,and relevant confounders were corrected,followed by subgroup analysis to analyze the correlation between them and FLD risk in different populations,and the interaction between them in FLD risk was further evaluated by Likelihood ratio test.Results: The serum TG concentrations of the control group and the FLD group were 1.48(1.17-1.72)mmol/L and 1.72(1.31-2.50)mmol/L,respectively.The serum TG concentrations of the FLD group were significantly higher than those of the control group(P < 0.001).Adjustment for gender,age,BMI,waist hip ratio,total cholesterol,high-density lipoprotein cholesterol,low-density lipoprotein cholesterol,alanine aminotransferase,fasting plasma glucose,uric acid,smoking,drinking,exercise,hypertension,family history of diabetes,compared with the group with TG ≤ 1.70 mmol/L,the risk of FLD was significantly increased in the group with TG > 1.70 mmol/L,and the Odds ratio(OR)and 95% Confidence interval(CI)was 1.68(1.12-2.52).In the subgroup analysis,the serum TG level was positively correlated with the risk of FLD in all subgroups except gender,hypertension and family history of diabetes.The minimum allele(G)frequency of PNPLA3 rs738409 locus in the control group and FLD group were 35.10% and 41.42%,respectively.The G allele frequency in the FLD group was significantly higher than that in the control group(P = 0.006).Compared with the C allele,after adjusting for the aforementioned confounders and TG level,the G allele significantly increased the risk of FLD,with an OR value and 95% CI of 1.33(1.06-1.66).Compared with CC genotype,GG genotype was significantly positively correlated with FLD risk,OR value and 95% CI were 2.36(1.31-4.26),but GC genotype was not correlated with FLD risk(P = 0.203).Subgroup analysis showed that the association between PNPLA3 rs738409 gene polymorphism and FLD was different in different characteristic populations.There was no significant interaction between serum TG level and PNPLA3 rs738409 gene polymorphism(P = 0.416).Conclusions: In this study population,high serum TG level and PNPLA3 rs738409 G allele were associated with increased risk of FLD.GG genotype increased the risk of FLD by 1.36 times,and there was no interaction between serum TG level and PNPLA3 rs738409 gene polymorphism.The results of this study suggest that both the person with high serum TG level and PNPLA3 rs738409 GG genotype are FLD high-risk population,which needs to be focused on,strengthen early screening and more accurate lifestyle intervention to prevent and control the occurrence and development of FLD.