The Correlation Between Vaginal Microecosystem, ILC3 And Cervical Lesions

Objective:1.To explore the relationship between vaginal microecological imbalance and HPV infection;2.To explore the relationship between the occurrence and development of cervical lesions,vaginal microecological imbalance and HPV infection;3.To analyze whether ILC3 is involved in the occurrence of cervical lesions.Methods:A total of 149 patients with cervical lesions who were treated in the hospital were enrolled in the study as experimental groups,including CINⅠ30 cases,CINⅡ37 cases and CINⅢ36 cases and 46 cases of cervical cancer.36 patients without HPV infection who underwent total hysterectomy due to benign diseases in our hospital during the same period were selected as the control group.HPV,TCT,vaginal microecology,and the expression of ILC3 cells in the cervical tissues of all subjects were detected.And the correlation among vaginal microecology,HPV,ILC3 and cervical lesions was analyzed.Results:1.With the severity of cervical lesions increased,The infection rates and of HPV16/18 decreased,and the difference was statistically significant(P<0.05).2.The PH value of the cervical lesion group was higher than that of the control group,but the difference was statistically non-significant(P>0.05).But there was difference in the abnormal PH value rates between the groups(P<0.05).The infection rates of BV increased with the increase of cervical lesion degree(P<0.05).3.The positive detectable rates of PH>4.5 and BV in HPV positive infection patients were higher than those in HPV negative infection patients,with statistically significant differences(P<0.05).4.With the increase of cervical lesions,the number of CD3~-IL17~+/CD3~-IL22~+cells decreased gradually,and the difference was statistically significant(P<0.05).5.There were more CD3~-IL17~+/CD3~-IL22~+cells in the cervical tissues with PH≤4.5than those with PH>4.5,and the difference was statistically significant(P<0.05).The number of CD3~-IL17~+/CD3~-IL22~+cells in the cervical tissue of BV infected patients was less than that of uninfected patients(P<0.05).The number of CD3~-IL17~+/CD3~-IL22~+cells in the cervical tissue infected with HPV16/18 was less than that in the cervical tissue not infected with HPV16/18,and the difference was statistically significant(P<0.05).Conclusion:1.Vaginal microecological imbalance promoted HPV infection.2.The occurrence and development of cervical lesions were related to the imbalance of vaginal microecology and HPV infection.3.ILC3 were involved in the immunity of the vagina,and its reduction or deletion lead to the occurrence and development of cervical lesions.