Studies On The Chemical Constituents Of Phellodendron Chinense And Yellow Tea(Camellia Sinensis L.)

Phellodendron chinense belongs to the genus of Phellodendron of the family Rutaceae,and it's dry bark is a traditional Chinese medicinal materia,which containsalkal oids,flavonoids,sterols,terpenes and other chemical components.P.chinense exh-ibits antibacterial,anti-inflammatory,antihypertensive,hypoglycemic,anticancer,immu-nosu ppressive,antifestering and other pharmacological effects.However,it is found tha-t th ere are few studies on the water-soluble constituents of P.chinense.Thus,the drbark of P.chinense was studied in order to provide basis for its further utilization.P.chine nse was extracted with 60% acetone water for three times,and then separated byHW-40,ODS,MCI and other chromatographic techniques.Finally,15 compounds wer-e is olated and identified,namely 3,5-dimethoxycinnamol-4-O-?-D-glucoside(1),coniferin(2),ligusinenside A(3),sinapadehyde glucoside(4),coniferialdehyde glucoside(5),quercetin(6),threo-3-methoxy-5-hydroxy-phenylpropanetriol-8-O-?-D-glucopyranoside(7),2-(4-hydroxylphenyl)ethyl-O-?-D-apiofuranosyl-(1–6)-O-?-D-glucopyranoside(8),benzoic acid,4-[(?-D-glucopyranosyloxy)methyl]-methyl ester(9),?-D-glucopyranoside,2-(4-hydrox yphenyl)ethyl(10),3-hydroxy-3,5-dimethoxyphenyl-?-D-glucopyranoside(11),(+)-magnofl orine(12),(+)-fuzitine(13),(+)-Menisperi-ne(14),3-hydroxy-3-methyl-5-oxy-5-((3,4,5-tri hydroxy-6-((7-hydroxy-1-(4-hydroxybenzyl)-2-methyl-1,2,3,4-tetrahy-droisoquinolin-8-yl)ox y)tetrahydro-2H-pyran-2-yl)methoxy)pentanoic acid(15).Among them,compound 15 is a new benzyltetrahydroisoquinoline-type alkaloid.Camellia sinensis(L.)O.Kuntze is a shrub or small tree of Camellia genus of Theaceae family.Yellow tea belongs to one of the six tea categories in China,which has a small reputation and is not known to the public.In recent years,scientists began to study yellow tea due to its unique efficacy.However,there are few studies on the water-soluble components of yellow tea.In order to provide the basis for the further utilization and development of yellow tea,we studied the 60% acetone water extract of yellow tea by HW-40,ODS,MCI and other chromatographic techniques.Finally,five compounds were identified as epigallocatechin gallate(1),epigallocatechin gallate(2),epigallocatechin(3),epigallocatechin(4),and epigallocatechin(5).