Research And Application Of Chemical Constituents And Biological Activity Of The Medicinal Plant Elsholtzia Vulgare From Guizhou

In this paper,the chemical constituents were extracted,separated and identified from the aerial parts of Elsholtziaciliata(Thunb.)Hyland in Guizhou province.Carry out biological activity measurement on that crude extract and the separated monocompounds of the it;The inclusion process of its volatile oil and β-CD was optimized.Volatile oils from different parts of fresh Mosla chinensis were extracted by steam distillation and solid phase microextraction respectively.the components and contents of volatile oils were determined by GC-MS.The results showed that the main components and contents of volatile oil were very different in different extraction methods,but the structure types of volatile oil were similar,mainly alcohols,aldehydes and ketones and terpenes.The main components of volatile oil in different parts of Elsholtzia were the same and the contents were different.The main components of volatile oil extracted by steam distillation were limonene(29.27%,23.20%),linalool(23.10%,21.05%),selinene(11.52%,7.78%),rosefuran(4.95%,5.56%).The main components of volatile oil extracted by solid-phase microextraction were(E,E)-α-farnesene(23.57%,10.32%),α-humulene(18.24%,9.13%),germacrene(15.46%,8.29%),α-naginatene(9.00%,16.43%),α-terpineol(8.75%,12.44%).Twenty compounds were isolated from the aerial parts of Elsholtziaciliata(Thunb.)Hyland.the structures of 14 compounds were identified by MS and NMR data.XR-1(tetracosane),XR-2(eicosanic acid),XR-3(β-sitosterol),XR-4(betulinic acid),XR-5(oleanolic acid),XR-6(5-hydroxy-6,8-dimethoxyflavone),XR-7(5-hydroxy-6,7-dimethoxyflavone),XR-8(5,7-dihydroxy-4’-methoxyflavonol),XR-9(5,7,4’-trihydroxyflavonol),XR-10(5,7,4’-trihydroxyflavonol),XR-11(5,7,4’-trihydroxy-5’-methoxyflavonol),XR-12(5,7,4’,5’-tetrahydroxyflavonol),XR-13(kaempferol-3-orutoside)and XR-14(isorhamnetin-3-o-neohesperidin),The compounds XR-1,XR-10,XR-11,XR-13,XR-14 were isolated from this genus for the first time.DPPH and ABTS methods were used to determine the antioxidant activity of the crude extract of this plant.The results showed that the crude extracts had DPPH and ABTS free radical scavenging activity,and the antioxidant activity of ethyl acetate was similar to that of BHT,but weaker than that of PG and BHA.The IC50 values of the free radical scavenging fractions were: ethyl acetate fraction(19.44μg.m L-1,31.34μg.m L-1),petroleum ether fraction(157.35μg.m L-1,329.54μg.m L-1),water fraction(24.98μg.m L-1,115.63μg.m L-1),Volatile oil fraction(37.88 mg.m L-1,10.22 mg.m L-1),PG(1.62μg.m L-1,5.95μg.m L-1),BHA(5.72μg.m L-1,13.82μg.m L-1),BHT(16.31μg.m L-1,20.11μg.m L-1).The relative order of scavenging free radical ability of crude extracts was ethyl acetate fraction >water fraction > petroleum ether fraction >volatile fraction.The bacteriostatic rate of the crude extract and mono-compounds was determined by UV spectrophotometry.the results showed that ethyl acetate and essential oil had better inhibitory effect on staphylococcus aureus.XR-12(5,7,4’,5′-tetrahydroxyflavonol)in the monomer had a better inhibitory effect on staphylococcus aureus than ampicillin.The inhibitory effect of water,petroleum ether and XR-6(5-hydroxy-7,8-dimethoxyflavone)on staphylococcus aureus was poor.The clathrate compounds of volatile oil β-CD was prepared by aqueous solution method,and the inclusion process was optimized by central composite design-response surface methodology.the results showed that the optimum conditions were as follows: the ratio of volatile oil to β-CD was 1:6.31(m L:g),the inclusion time was 242 min(4h),and the inclusion temperature was 59.8℃.According to the optimization results,three groups of validation tests were conducted in parallel.the deviations of oil content Y1,yield Y2 and inclusion rate Y3 from the predicted values were 1.56%,1.89% and 0.98%,respectively.The actual value is close to the predicted value,which indicates that the reliability of the optimized process parameters is high.The clathrate compoundswas characterized by UV,IR,DSC and SEM.the results showed that β-CD clathrate compounds of Elsholtzia volatile oil had been formed.The study can provide a theoretical basis for the further development and utilization of medicinal plant resources in Guizhou province.