| Objective:To explore the effect of edible and medicinal Chinese herbal medicine on dyslipidemia and its effect on vascular endothelium based on the literature study and animal experimental studyMethods1.Literature research:Search the clinical literatures about dyslipidemia in CNKI,Wanfang and Weipu datebases in recent 10 years.And then using the auxiliary platform V2.5 of traditional Chinese medicine to set up the database of dyslipidemia.Labeling and extracting edible and medicinal Chinese herbal medicine based on the catalog-110 kinds of edible and medicinal Chinese herbal medicine published by the Health Commission in 2018.The Frequency and association rules of the data were analyzed,and the four-qi,five-flavor,channel tropism and pharmacodynamics were analyzed statistically.2.Experimental research(1)48 Wistar rats were randomly divided into 6 groups(N=8):blank group,model group,low-dose group,medium-dose group,high-dose group and positive control group,and the rats in each group were fed and drank freely.The levels of Serum Lipid(TC,TG,HDL-C,LDL-C)in each group were measured to confirm the stability of blood lipid baseline before drug intervention.(2)From the second week,the blank group was given 20 ml/kg/d distilled water in the morning(8:00 am)and afternoon(3:00 pm),the model group was given 20ml/kg/d high-fat emulsion in the morning and the same amount of distilled water in the afternoon,and the intervention groups were given high-fat emulsion in the morning,and the rats were given the corresponding drugs(5.625g/kg/d,11.250g/kg/d,22.500g/kg/d,4.167mg/kg/d,respectively in the low,middle and high dose groups and the positive control group)in the afternoon.After the dyslipidemia model was established,the levels of Serum Lipid(TC,TG,HDL-C,LDL-C)were measured in each group to determine whether the dyslipidemia rat model was established,and the to further explore the preventive effect of edible and medicinal Chinese herbal medicine on dyslipidemia.(3)From the 6th week,the blank group and the model group were given intragastric infusion of distilled water at the above dose.The low,middle and high dose groups and the positive control group were given intragastric administration at the above dose respectively.The positive control group was given intragastric administration of Atorvastatin at the above dose once a day(8:00am),four weeks of continuous treatment.After treatment,blood,liver and thoracic aorta of rats were collected to investigate the therapeutic effect of edible and medicinal Chinese herbal medicine on dyslipidemia and its effect on vascular endothelium.Results1.Literature research resultsA total of 468 prescriptions were involved in 432 literatures,including 209 Chinese herbs,of which 63 were edible and medicinal Chinese herbal medicine.There were 29 edible and medicinal Chinese herbal medicine with the herbs whose frequency≥10(accounting for about 46.03%of all),and the frequency of occurrence is 1661 within the herbs whose frequency≥10(accounting for about 94.91%of all).Among the most frequently used drugs were hawthorn,tuckahoe,cassia seed,astragalus,licorice,gngelica,lotus leaf,peach kernel and so on.The most frequent Qi is steady in the four Qi,accounting for about 44.83%.The most frequent taste is sweet taste in five tastes,accounting for about 43.48%.The frequency meridians is spleen in the all meridians,accounting for about 23.46%.The use of drugs almost from the spleen,liver,kidney,and pay much attention to removing dampness and phlegm,blood stasis,and regulating Qi.Extracted 10 pairs of edible and medicinal Chinese herbal medicine,respectively:(hawthorn,cassia seed),(hawthorn,tuckahoe),(hawthorn,astragalus),(tuckahoe,cassia seed),(hawthorn,tuckahoe,cassia seed),(hawthorn,lotus leaf),(hawthorn,licorice),(tuckahoe,licorice),(tuckahoe,astragalus),(cassia seed,astragalus),a total of 6 herbs were involved,namely hawthorn,cassia seed,tuckahoe,astragalus,lotus leaf,licorice.2.Experimental research results(1)The determination of the blood lipid level in each group of rats with stable baseline and the establishment of dyslipidemia modelThere was no significant difference in TC,TG,HDL-C and LDL-C(P>0.05),which indicated that the baseline of blood lipid level was stable.After 4 weeks,the levels of TC and LDL-C in the model group were significantly higher than those in the blank group(P<0.01).(2)The preventive effect of edible and medicinal Chinese herbal medicine on dyslipidemia①The results showed that the level of TC in each group was significantly lower than that in the model group(P<0.05),and there was no significant difference between the two groups(P>0.05).The level of TC in low and high dose groups,the level of LDL-C in three dose groups and western drug group were decreased,but there was no significant difference(P>0.05).②The results of serum lipid levels before and after model making showed that TC and LDL-C in model group,edible and medicinal Chinese herbal medicine low-dose and high-dose groups and positive control group were significantly higher than those before model making(P<0.01).And the level of TC and LDL-C in edible and medicinal Chinese herbal medicine medium-dose group increased significantly(P<0.05).③The results of the difference before and after the modeling showed that,compared with the model group,there was no statistical difference in the difference of serum lipid indexes in the drug intervention group at the levels of TC and LDL-C(P>0.05).(3)Therapeutic effect of edible and medicinal Chinese herbal medicine on dyslipidemia①After treatment,the results of blood lipid levels of rats in each group showed that compared with model group,the TC level of each drug intervention group decreased,among which the low and high dose groups of edible and medicinal Chinese herbal medicine changed significantly(P<0.05),the middle dose group and the positive control group changed extremely significantly(P<0.01),there was no statistical difference between the low and high dose groups of drug food homology,the middle dose group of drug food homology and the positive control group(P>0.05),and the TC level of the latter two groups was significantly lower than that of the former two groups(P<0.05).The level of LDL-C in the middle,high dose and positive control groups was significantly lower(P<0.01),and there was no statistical difference among the three groups(P>0.05).Although LDL-C level in the low dose group was lower,there was no statistical difference(P>0.05),and LDL-C level was significantly higher than that in the other three groups(P<0.01).②Compared with before treatment,there was no statistical difference in the levels of TC and LDL-C in the model group after treatment(P>0.05).The levels of TC in the low-dose,medium-dose,high-dose and positive control groups were significantly reduced(P<0.05,P<0.05,P<0.01,P<0.01),and the levels of LDL-C in the above four groups were also significantly reduced(P<0.05,P<0.05,P<0.05,P<0.01).③Comparison of the difference before and after treatment showed that,compared with the model group,the difference values of TC and LDL-C in the middle,high dose and positive control groups were significantly increased(P<0.01).The difference of TC in the low-dose group was also increased(P<0.05),and the difference of LDL-C in this group was significantly lower than that in the other three groups(P<0.01).(4)The effects of edible and medicinal Chinese herbal medicine on vascular endotheliumCompared with the blank group,the vascular endothelial structure in the model group was seriously damaged.The damage of each drug intervention group was lower than that of the model group,and the medium dose group with Chinese medicine food homology had the least pathological changes,followed by the positive control group,and the high and low dose group with medicine and food homology.The statistical results of biochemical indicators related to vascular endothelial function were as follows:①Compared with the model group,the level of NO in the middle and high dose edible and medicinal Chinese herbal medicine group and the positive control group was significantly increased(P<0.01),the low-dose edible and medicinal Chinese herbal medicine group was significantly lower than the other three groups(P<0.01),and the medium-dose edible and medicinal Chinese herbal medicine group was significantly higher than the positive control group(P<0.05).②Compared with the model group,the level of ET-1 in all the drug intervention groups decreased significantly(P<0.01),and the low-dose group of edible and medicinal Chinese herbal medicine was significantly higher than that of the middle-dose group,high-dose group and positive control group(P<0.01).③Compared with the level of TNF-α in the model group,the level of TNF-α in each drug intervention group was significantly lower(P<0.01),in which the low dose of drug food homology was significantly higher(P<0.05,P<0.01,P<0.01)than that in the middle-dose group and the positive control group(P<0.01,P<0.05).④Compared with the level of IL-6 in the model group,the level of IL-6 in the low,middle and high dosages group and the positive control group were significantly lower(P<0.05,P<0.01,P<0.01),and the level of IL-6 in the low,middle and high dosages group was significantly higher(P<0.01),the level of IL-6 in the Western dosages group was significantly lower(P<0.01),the level of IL-6 in the middle and high dosages group was significantly lower(P<0.01),the level of IL-6 in the Western dosages group was significantly lower(P>0.05).⑤Compared with the MDA level of the model group,the MDA level of the positive control group decreased significantly(P<0.01).In addition,low-dose group was significantly lower than middle,high dose group and positive control group(P<0.01).⑥Compared with the model group,the level of SOD in the middle and high dose groups and positive control group was significantly increased(P<0.01),and the SOD activity in the serum of the three groups was far lower than that in the low dose group(P<0.01).Conclusion1.Conclusion of Literature Research In clinical intervention of dyslipidemia,edible and medicinal Chinese herbal medicine take care of the five viscera,and It mainly involves the liver,spleen and kidney,especially pay attention to promoting blood circulation and removing blood stasis,removing dampness and phlegm,and regulating Qi.Therefore,phlegm turbid and blood stasis is the important pathological factor of dyslipidemia,and the mutual accumulation of phlegm and blood stasis is the basic pathogenesis.Removing blood stasis and resolving phlegm can be used as the guiding principle of medicine and food homology to intervene dyslipidemia.2.Conclusion of the Experimental Research In the stage of prevention and treatment of dyslipidemia,edible and medicinal Chinese herbal medicine based on the combination of phlegm and blood stasis can play a good role in the prevention and treatment of dyslipidemia,and it is preliminarily believed that the protection of vascular endothelium by edible and medicinal Chinese herbal medicine based on phlegm and blood stasis may be related to the improvement of vasomotor state,anti-inflammatory and anti-oxidation. |
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