The Role Of IL-6/STAT3 Signaling Pathway In The Intestinal Mucosal Immune Barrier Of UC Rats With Damp-heat Syndrome And The Intervention Mechanism Of Shaoyao Decoction

Objective:This study is based on Shaoyao Decoction to explore the mechanism of Shaoyao Decoction on IL-6/STAT3 signaling pathway and the effect of Shaoyao Decoction on the intestinal mucosal immune barrier damage of UC rats with internal accumulation of damp-heat by interfering with IL-6/STAT3signaling pathway mechanism.Methods:The UC rat model of damp-heat internal accumulation syndrome was established,and the experimental group rats were given different tested factors.The expression of COX-2 in colon tissue was detected by observing the rat’s general behavior,HE staining pathological sections and immunohistochemical method to evaluate the damp-heat type UC.Whether the mouse model is successful.The immunohistochemical method was used to detect the expression of IL-6,STAT3,CD4~+,and CD8~+in colon tissue,so as to explore whether Shaoyao Decoction can repair the intestinal mucosal immune barrier by interfering with the expression of IL-6/STAT3.Results:(1)Rat general behavioral assessment,rat colon Luketal score,colon histopathological score:Compared with the blank group,the DAI score,Luketal score,and colon histopathological score of the model group were significantly increased after modeling(P<0.01).Compared with the blank group,the DAI score,Luketal score,and colonic histopathological score of rats in each administration group increased to different degrees(P<0.05).(2)COX-2expression in colon tissue:COX-2 was the highest in the model group and lowest in the blank group.There were significant differences in the other treatment groups(P<0.05).(3)The expression of IL-6 and STAT3 in colon tissue:The expression of IL-6 and STAT3 in colon tissue in the model group was the highest(x±s=6.00±2.09,x±s=5.71±2.02).The expression level of IL-6 in colon tissue of the inhibitor group+Shaoyaotang group was not significantly different from that of the blank group(P=0.14>0.05,P=0.07>0.05).(4)The expression of CD4~+and CD8~+T cells in the colon tissue:The expression of CD4~+and CD8~+T cells in the colon tissue of the model group and each treatment group was lower than that of the blank group(P<0.01).Conclusion:(1)Shaoyao Decoction can inhibit IL-6/STAT3 signaling pathway and inhibit the expression of COX-2.(2)Shaoyao Decoction can increase the expression of CD4~+and CD8~+T cells in the colon tissue of rats.(3)IL-6/STAT3signaling pathway may not be the main target of mesalazine.(4)Shaoyao Decoction may increase the expression of CD4~+and CD8~+T cells in rat colon by inhibiting the expression of IL-6/STAT3 signaling pathway.