Jiawei Shaoyao Decoction In The Treatment Of Ulcerative Colitisclinical Curative Effect Observation And On TLR2,4/NF- κB Signal Pathway In Experimental Rats

Objective:To observe the effect of Jiawei Shaoyao Decoction on the clinical function in ulcerative colitis patient (large intestine damp-heat type and), the pathological variety of the chronic ulcerous colonitis rats’mucous membrane, and the influence of TLR2、TLR4/NF-κB To discuss the underlying mechanism’s action of the treatment of ulcerative colitis, and the study provide theoretical basis for the treatment of chronic ulcerous colitis.Methods:Clinical study:According to the principle of randomization and contrast, 62 patients were divided into two group, the control group (30 patients) and the reatment group (32 patients) The control group was treated with mesalazine, lg/time, three times a day. The treatment group patients was given Shao Yao Tang (p. o.) at the basic of control group. The total period of treatment is thirty days. Observed the variety of the symptoms, signs, and the mucous membrane’s under the colonoscopy between the before-treatment and after-treatment, then score them. Experimental part:The UC rat models were established by the method of Trinitrobenzene sulfonic acid (TNBS) or alcohol, and then be divide into 4 groups, as follows:normal control group, model control group, ShaoYao Tang Presciption dose group, and SASP group. Immunohistochemical method for observation of the levels of the intestinal mucosa’s TLR2、TLR4 and NF-κB after the treatment.Results:Clinical part:After the treatment, the treatment group’s score declined obviosly than the control group’s, the total efficacy rate of the two groups at tenesmus the efficacy of TCM are 94.15% and 89.2%, the treatment group better than in the control group. The treatment group has the advandge at the improvement of abdominal pain, diarrhe and anal burning (P<0.05),but at the impact of improving hematochezia, the two groups are identical. Also, there was no adverse events between the two groups.Experimental part:During the test, the blank group eat food normally, with coat gloss, full of vitality, and sensitive response, stool is particles and the anus’s around was clean. In the model group:after using Trinitrobenzene sulfonic acid (TNBS) or alcohol established, the UC rat models’action became poorly and slowly, dietary reduction, stool thinning at that day. Mice appear bloody stools, even seen dark brown biood in the stool, fur messy little luster, dirtiness around the anus, easy frightened, like to stay together in the 2nd day. The Jiawei Shaoyao Decoction group and the SASP group were given the medicine, the sydromes become better slowly, the fur became shiny, drinking and eatting bebame much more, lessness blood stool and diminished basicly, the shape of stool was becoming forming, responsive and growing more heavy.2. HE staining:the blank group mice colon mucosa showed the complete structure, non obvious inflammatory response, the modle group:the lood vessels of mucosa and submucosa mainly were expansion with blood, lots of neutrophil, lymphocyte and plasma cells infiltration, damagement of the glands’structure. After the 14 days’treatment, the Jiawei Shaoyao Decoction group and the SASP group shows that, the inflation with blood of mucosa reduced obviously, little neutrophil, infiltration, and the long glands. The score of the Jiawei Shaoyao Decoction group is lower than the SASP group’s.3. The immunohistochemistry shows:the TLR2、TLR4 and NF-κB expression were significantly increased in the modle group, after the treatment, the inflammation evaluation index were significantly decreased, the TLR2、TLR4 and NF-κB expression were significantly declined. The TLR2、TLR4 and NF-κ B expression of the Jiawei Shaoyao Decoction group was less than the SASP group’s. There was no significant (P<0.05).Conclusion:The effect of the Jiawei Shaoyao Decoction & Mesalazine on the large intestine damp-heat type ulcerative colitis is obviously Better than the singal west medcine group, and obvious improvment at the sydromes. TLR2., TLR4 and NF-κB in the UC model mice increased their erexpression, which can lead to ulcerative colitis worsen. Jiawei Shaoyao Decoction can reduce the expression of TLR2、TLR4 and NF-κB in the UC, by blocking the signaling pathway of TLR2、TLR4/NF-κB.