Study On The Effect Of Sunflower Plate Hydrolyzate On Blood Lipid And Intestinal Flora In Mice With Hyperuricemia

The increase of blood uric acid level in the human body not only causes abnormal blood lipid metabolism,but also has a disorder of intestinal flora structure.Currently used to treat hyperuricemia,there are limitations or serious side effects.In this study,the effect of the sunflower plate hydrolyzate(SPH)on the blood lipids and intestinal flora of hyperuricemia model mice was studied in order to more fully clarify its effects.At the same time,it can also explore the relationship between hyperuricemia,blood lipids and intestinal flora.In this study,mice with hyperuricemia model were first established,and then mice with hyperuricemia were randomly divided into 5 groups.The model group,allopurinol group,SPH high dose group,SPH medium dose group and SPH low dose group were named according to the type and dose of the drug to be administered,and the normal group was established at the same time.During the test,changes in body weight,food intake and other indicators of each group of mice were collected.After the end of the experiment,the serum and liver of mice were collected.Determination of serum uric acid level(UA),total cholesterol level(TC),triglyceride(TG),low density lipoprotein(LDL-C),high density lipoprotein(HDL-C),alanine aminotransferase(ALT),and cereal Transaminase(AST).At the same time,the Hiseq 2500 platform was used to sequence the fecal microbial gene of each group.Serum biochemical indicators showed that the UA level in the model group was significantly higher than the other groups,indicating that the hyperuricemia model was stable during the experiment.The difference in TC content was not significant between the groups of mice.There was no significant difference in TG content between the blank group and the remaining groups;but there was a significant difference between the model group and the SPH groups.The HDL-C content of the model group mice was lower than SPH groups and blank group.The LDL-C content in the blank group was slightly higher than that in the model group,allopurinol group and SPH low dose group.The SPH high and medium dose groups were slightly higher than the blank group,but there was no significant difference compared with the blank group.The Elisa method showed that compared with the blank group,the ALT and AST contents in the model group were higher,and there were significant differences.There was no significant difference between the allopurinol group and the SPH group.Compared with the model group,there was a significant difference in ALT content in the high-dose SPH group;there was a significant difference in AST content between the blank group and the SPH group.High-throughput sequencing results show that both the sparsity curve and the equal-abundance curve tend to be flat.This indicates that the library capacity is large enough,the sample size is sufficient,and the difference between animal groups is small.The experiment found that the most abundant intestinal flora of the mice in this study was Bacteroidetes.Bacteroidetes are more abundant in mice with SPH than in the blank group,and Proteobacteria was significantly reduced.Compared with the model group,Bacteroidetes were significantly increased in each dose group of SPH,and Firmicutes was significantly reduced.After principal component analysis,principal coordinate analysis,non-metric multidimensional scale analysis and other methods,it was found that the intestinal flora sequencing results of each group of mice were smaller within the group.The LEf Se analysis found that the most significant species were the model group.The SPH high,medium and low dose groups were analyzed and compared,and the species with significant differences in the SPH low dose group were found to be the most.The differential genes in the sputum metabolic pathways of the blank group,the model group,the allopurinol group and the SPH high-dose group were compared and analyzed.The homologous genes with the highest relative abundance in each group were found to be 42 species,12 species and 5 species.,45 species.In summary,the intervention of SPH will improve the blood lipid index of mice with hyperuricemia model to a certain extent,and the related indicators tend to return to normal levels.And SPH can improve the liver function of mice with hyperuricemia to a certain extent,which is beneficial to the lipid metabolism of mice.SPH can also alter the intestinal flora structure of hyperuricemia model mice,affecting the total number of species,phylogenetic diversity,microbial diversity(abundance,uniformity)of the intestinal flora of hyperuricemia model mice.And metabolic processes such as sputum metabolism in mice with hyperuricemia.SPH can also affect metabolic processes such as purine metabolism in hyperuricemia mice.