| Objective:To observe the clinical efficacy of Qigong Pill in the treatment of patients with phlegm-dampness polycystic ovary syndrome(PCOS),and to establish rat model of PCOS phlegm-dampness syndrome.To observe and analyze the effect of Qigong Pill on AMH / AMHRII expression and autophagy in ovarian granulosa cells of PCOS phlegm-dampness syndrome rats,and to clarify the possible mechanism of Qigong Pill in the treatment of PCOS phlegm-dampness syndrome.Method:In the clinical research part,a total of 50 patients with PCOS(phlegm-dampness type)were collected by retrospective study.The changes of sex hormone levels,body mass index(BMI)and TCM symptom scores before and after treatment were analyzed.In the basic research part,the mechanism of Qigong Pill regulating the autophagy of rat granulosa cells through AMH / AMHRII pathway to treat PCOS phlegm-dampness syndrome was discussed,and the rat model of PCOS phlegm-dampness syndrome was established.Thirty experimental rats were randomly divided into normal control group,model group,traditional Chinese medicine group,western medicine group and traditional Chinese medicine + western medicine group.The pathological sections of rat ovaries were observed by HE staining.The contents of luteinizing hormone(LH),testosterone(T)and insulin(Ins)in serum were detected by ELISA.The distribution of LC3 in ovaries was detected by immunohistochemistry.Ovarian vessels were labeled with CD31 to observe the changes of ovarian vessels.The expression levels of AMH,AMHRII,Beclin1,LC3 A / B and p62 were detected by Western blot.Result:Clinical research part :1.BMI : Compared with before treatment,BMI decreased after Qigong pill treatment,and the difference was statistically significant(P <0.05).2.TCM symptom score : After treatment,the individual scores and total scores of TCM symptoms were lower than those before treatment,and the difference was statistically significant(P < 0.05).3.Sex hormone level : FSH level after treatment was not statistically significant compared with that before treatment(P > 0.05),and the levels of LH,LH / FSH and T in patients after treatment were lower than those before treatment,and the difference of LH and LH / FSH ratio before and after treatment was statistically significant(P <0.05),while the difference of T was not statistically significant(P > 0.05).4.Comprehensive efficacy :The total effective rate after treatment was 82.98 %.Basic research part :1.ELISA results : The levels of LH,T and Ins in the serum of the model group were significantly higher than those in the normal control group(P < 0.001);the contents of LH,T and Ins in Chinese medicine group,western medicine group and Chinese medicine + western medicine group were significantly lower than those in model group(P < 0.001).2.HE staining : Pathological sections showed that dominant follicles and corpus luteum were seen in the ovaries of rats in the normal control group,and polycystic changes were seen in the ovaries of rats in the model group.The number of immature follicles in the Chinese medicine group,western medicine group and Chinese medicine + western medicine group was lower than that in the model group,but higher than that in the normal control group.3.Immunohistochemistry : The expression of LC3 in the model group was significantly higher than that in the normal control group;the expression of LC3 protein in Chinese medicine group,western medicine group and Chinese medicine + western medicine group was lower than that in model group.4.Immunofluorescence : Compared with the blank group,the rats in the model group had obvious ovarian vascular proliferation,rich blood flow,and increased CD31 expression,while the proliferation of blood vessels in the Chinese medicine group,the western medicine group,and the Chinese medicine + western medicine group was inhibited compared with the model group.5.Western blot : The expression of AMH,AMHRII,Beclin1 and LC3 A / B in ovarian granulosa cells of the model group was significantly higher than that of the normal control group(P < 0.001),and the expression of p62 was significantly decreased(P < 0.001).Compared with the model group,the expression of AMH,AMHRII,Beclin1 and LC3 A / B in ovarian granulosa cells of the Chinese medicine group,the western medicine group and the Chinese medicine + western medicine group was significantly decreased(P < 0.001),and the expression of p62 was significantly increased(P < 0.001).Conclusion:1.Qigong Pill can reduce the BMI level of PCOS patients with phlegm-dampness syndrome,improve their sex hormone disorder and relieve their clinical symptoms.2.Qigongfang can down-regulate the expression of autophagy-related proteins Beclin1 and LC3A / B through the AMH / AMHRII signaling pathway,inhibit the excessive autophagy of ovarian granulosa cells,thereby improving the ovarian morphology of PCOS rats,improving their sex hormone levels,and restoring normal ovarian function. |
PDF Full Text Request