Studies On Chemical Compositions And Pharmacological Activity From Fruitbodies And Culture Fluid Of Gloeostereum Incarnatum

The chemical components Were analyzed systematically from fruitbody and culture fluid of Glocuslereum incarnation, their pharmacological activity and toxicological experiments were investigated deeply.Systematic pre-experiment showed that fruitbody of G.incarnatum contained polysaccharides, amino acid, flavonoside. triterpene glycoside, alkaloidse. sterol. fatty acid.Fruitbody and culture fluid of G incarnatum contained microelements Fe, Cu. Zn. Ca, Mg, Mn. By MC/MS, 9 kinds of etherdissolution substances were identified from fruitbody of G incarnatum, most of them were fatty acid, that were Dodecanoic. Tetradecanoate, 9-Methyltetradecanoate, Pentadecanoic acid, 7-Hexadecenoic acid, Hexadecanoic acid, 10,13-Octadecadienoic acid, 2',4'-Dimethoxy-3'-methylpropiophenone. Heneicosanoic acid, by same way, 6 kinds of etherdissolution matter were identified from its culture fluid, most of them possessed benzene ring, this fatty acid were Benzeneacetic acid, Benzenepropanoic acid, Dimethyl phthalate, Nonanedioic acid. Diphenylamine, Acetamide, N,N-diphenyl-. Fatty acid of fruitbody and culture fluid from G incarnatum, were not similar.A new component 6'-cl-l - 6'-0-saccharose was identified from culture fluid of G incar nar turn.The water solube crude polysaccharide had been exacted by HCl(pH=2 FS- 1 ). Carbamide(6% FS-II). CC13COOH(FS-III) from Gloeostereum. insarnatum. S.I toet 1 mai(G.LCF8511), The crude polysaccharide FS- I , FS- II and FS-III were analysed by G.C, They are consist of XyK Man, Glc.The molar ratio is 1.3:1:1 in FS- I , the ratio is 2.2:2:1 in FS-II. and also it is 2:1.4:1 in FS-III. Total polysaccharides GP were also isolated from culture fluid of G. incarnatum. The molar ratio of Man:Glc is 1:9.FS-III had been fractionated by ethanol. Three fractions:FS-IIla, FS-IIIb, FS-II1C The struction of FS-1IIC was determined in this experiment. FS-II1C is brown power. G.C analysis indicate that FS-IIIC is composed of Man and Glc. The molar ratio is 1.42:1. Through Sepharose CL-6B gel filitration and cellulous acetate electrophoresis. FS-III, is determined to be homogeneous.The totle content of FS-IIIC is 96%, Through Sepharose CL 6B gel filtration, itsaverage molecular weight is 126KD.Through IR, G.C, partial hydrolysis with acid, periodate oxidation. Smith degradation, methylation and G.C-MS analysis .They reveal that FS-111C is a kind of lowly branched structure. The main chain is mainly made up of Man, Glc, |3 -(1-3) Man and 3 -(1-3) Glc, P -(1 -3) Glc resudues are substituted at 6-O.On average, there are 2 branches among 11 main chain residues. The side chain is composed of (1 -*4) Glc. The nonreduced end is composed of Man > Glc.Pharmacological activity and toxicological experiments of total polysaccharides GP had been isolated from culture fluid of G. incarnalum showed that 1. Water extract and GP from Gloeostereum insarnalum inhibited the growth of bacteria. 2. GP could strengthen non-specific immunity of mice. 3. GP could alleviate mice ears plumping caused by xylene.4. GP could alleviate rats legs plumping caused by carrageen colloid.5 Effect on rats Legs plumping induced by in different components from culture fluid of G. incarnatum. 6. GP had anti-ulcer effects. 7. GP could protect obviously intestine mucosa induced by acetic acid in rats. 8. GP could ameliorate spleen asthenic symptom induced by reserpine in mice. 9. The toxicological exoeriments of short-term and long-term showed that GP had no toxicity by per os.The results in pharmacological activity and toxicology of GP showed that GP can be exploited into a new drug, this drug can be used in acute enteritis and ulcerative colitis.