The Preparation Of Granule Lecithin And High Phosphatidylcholine Content Lecithin And Study On The Surface Physical Properties

The Phospholipids with high content Phosphatidylcholine was obtained using Acetone and Ethanol as solvents, emulsifying extraction technique, and wet gum Phospholipids as raw materials. The extraction conditions were optimized. A full factorial experiment method and a second-order orthogonal design were used in the study. The regression equations were calculated for maximum value of the response functions optimized. The main content in this thesis are:1. The granule Soya lecithin was purified by deoiling with acetone from Soya wet gum. The extraction parameters were studied. Through orthogonal design the optimum conditions were determined. Under the condition of using anhydrous acetone, Soya wet gum (g) : solvent Soya (ml) is 30 = 250, temperature is 12C and extraction time is 2 min, lecithin can be made into granule Soya lecithin, which acetone insoluble can reach 98.5%.2. Soybean phospholipids with high content of phosphatidylcholine was fractionated with ethanol, and optimum conditions have been determined to improve phosphatidylcholine(PC) enrichment. The effects of extraction time, solvent volume, ethanol concentration and temperature on the yield and PC enrichment have been described. A full factorial experiment method and a second-order orthogonal design were used in the study. The use of calculated optimal parameters in the fractionation process led to 69.65% PC enrichment and the yield of 25.6% , respectively.3. Based on the first-step extracting with ethanol as solvent, a second extracting with the same solvent was experimented. The availability of this method was proved. And PC content of the extracts was increased from 54.48% to 76.68%. The PC content about 90% of Phospholipids could be obtained using extracts of ethanol as raw material.4. Surfacial properties of Soybean lecithin with different PC content were studied in this paper. The conductivity of water solution (or emulsion) of phospholipids was measured and the changing was observed when ethanol added to the solution (or emulsion). Wetting power and emulsifying ability of lecithin were also measured. Stability of emulsions of soybean oil-lecithin-water was studied in which phospholipids were used as emulsifier, and it was found that stable emulsion required more of emulsifier when electrolyte(NaCl) was added to the emulsion.5. Soybean phospholipids were analyzed by positive-phase high performance liquid chromatography using gradient eluted program and evaporative light-scattering detector. The quantitative analysis of the four important components PC,PE,PI,PA of soybean phospholipids has been achieved with external standard method. The relative standard deviations is 0.82% ~ 1.34%, The recovery of the method is 96.7% ~ 100.8%. This method has been applied to the analysis of soybean phospholipids.