| Environmental Endocrine is a kind of exogenous chemicals which can disorder endocrine systems of humans and other organisms. Researches have shown that even trace amount of Environmental Endocrine is harmful to human body. Therefore, the analysis and measurement of Environmental Endocrine are of great practical and social significance.Molecules of intermediate or product can absorb chemical energy, transit from the excited state to the ground state, and produce optical radiation phenomenon. Chemiluminescence is an analytical method for quantitative and qualitative analysis by utilizing the produced optical radiation without the excitation of any light, electricity, magnetism, sound or heat sources. Due to its excellent advantages, such as high sensitivity, wide linear range, fast analysis speed, simple device, and low cost, chemiluminescence analysis method has been widely applied in various trace and ultra-trace analytical fields. However, the poor selectivity limits its application in the analysis of complex samples.Molecular imprinting technique (MIT) is a newly developed method to prepare polymers with specific recognition ability to target molecules. With advantages of similar identification property as natural antibody and excellent corrosion resistance as macromolecules, molecularly imprinted polymers (MIPs) have shown excellent application prospect in various fields.In this paper, molecular imprinting technique was combined with chemiluminescence analysis to determinate pollutants of Environmental Endocrine. Environmental Endocrine molecules were selectively enriched on the MIPs and followed by chemiluminescence detection. Taking advantage of the specific identification and capture ability of MIPs, the selectivity and sensitivity of chemiluminescence analysis were improved for the detection of Environmental Endocrine Disruptors (EEDs).The first section of the paper is the summary of literatures.In chapter one, the research status of Environmental Endocrine Disruptors was systematically summarized. An overview of different chemiluminescence systems and their analytical applications were provided. In addition, fundamental principles of molecular imprinting technique, preparation method as well as application of molecularly imprinted polymers were introduced.The second section of the paper is research report, including chapter two, chapter three, and chapter four.In chapter two, bisphenol A (BPA) molecularly imprinted polymer was prepared byPickering emulsion polymerization and applied in solid phase micro-extraction for selective enrichment of BPA. By combining with flow injection analysis, a MIP-chemiluminescence method was developed for BPA determination based on its inhibiting effect on Luminol-KIO4-NaOH system. The linear range of this method was 0.05-1.50 mg/L and 1.50-15 mg/L with the detection limit (LOD) of 17 ?g/L and the relative standard deviation (RSD) of 4.6% for six times.In chapter three, pyrocatechol molecularly imprinted polymer was prepared by Pickering emulsion polymerization and employed to prepare a solid phase micro-extraction column for the selective enrichment of pyrocatechol. By combining with flow injection analysis, a MIP-chemiluminescence method was developed for pyrocatechol determination in water based on its inhibiting effect on Luminol-K3[Fe(CN)6]-NaOH system. The linear range of this method was 0.01-0.1 mg/L and 0.1-3.0 mg/L with the detection limit (LOD) of 3.3 ?g/L and the relative standard deviation (RSD) of 2.6% for six times.In chapter four, diethylstilbestrol molecularly imprinted polymer was synthesized by magnetic surface molecular imprinting technique. The diethylstilbestrol-MIP was immobilized by an external magnetic field and used to prepare a solid phase micro-extraction column for the selective enrichment of the diethylstilbestrol. By combining with flow injection analysis, a MIP-chemiluminescence method was developed for diethylstilbestrol determination in water based on its inhibiting effect on Luminol-H2O2-NaOH system. The linear range of this method was 0.01-0.5 mg/L and 0.5-5 mg/L with the detection limit (LOD) of 3.3 ?g/L and the relative standard deviation (RSD) of 2.4% for six times.In this paper, molecularly imprinted polymers of three environmental hormone pollutants were synthesized and used as recognition elements in chemiluminescence determination. The developed MIP-chemiluminescence method showed advantages of high sensitivity and excellent specific recognition ability. This method was applied to real-time, on-line and specific determination of environmental hormone pollutants in real water samples. Furthermore, this method can provide guidance for the research of monitoring environmental hormone molecules in water environment. |
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