Study On Analysis Of Pharmaceutical And Pesticide Residue Using Flow Injection-Chemiluminescence

Chemiluminescen analysis (CL) can provide a high versatility in the determination of a wide variety of organic or inorganic substances, biological substances that exhibit bioactivities even at extremely low concentrations along with high sensitivity, wide linear range, rapidity, simplicity, which require only simple and low-cost-measuring devices. The noise from CL is much less than fluorescence method, because its excitation doesn't need any light source. A substance, which can affect CL reaction including the change of intensity, rate, wavelength, can be determined by CL method. It is introduced in this article the history of CL development, essential principle, most reaction system, some coupling techniques with CL, including flow-injection analysis, electrochemistry, molecularly imprinted polymer, high performance liquid chromatography, capillary electrophoresis, and our research work on the application of FI-CL, with an emphasis on the determination of some pharmaceuticals and the residue of pesticide based on alkaline luminal CL system, NBS CL system, acidic Ce(â…£)-NaSO₃ CL system, KMnO₄-HCHO CL system, KMnO₄-Rh 6G CL system, using a FI-CL method. The possible mechanisms of some CL reactions were preliminarily discussed using experiment and references. The main content of this article is as follows:1. It was found that the weak chemiluminescence produced from the reaction of dobutamine hydrochloride with luminol in alkaline solution could be strongly enhanced by potassium ferricyanide and potassium ferrocyanide. Based on this found, a new flow injection chemiluminescence method is proposed for the determination of dobutamine hydrochloride. Under the optimum conditions, the proposed method has a linear range of 1.0 ×10^-10 g ml^-1 to 1.0 ×10^-7 g ml⁽-10, with a detection limit of 2.6 × 10^-11 g ml^-1 for dobutamine hydrochloride (3σ ). A complete analysis could be performed in 40 s including washing and sampling, giving a throughout of about 90h^-1 . The relative standard deviation (RSD) for 11 parallel measurements of 1.0 ×10^-8 g ml^-1 dobutamine is 1.23%. The proposed method has been applied for the...