Preparation And Applications Of Poly (Vinyl Ester Resin) Monolithic Columns

Development of new style chromatographic packing materials and improvement of their performance are important in the study of chromatography. First, a new monolithic column of poly(vinyl ester resin-co-ethylene dimethacrylate) has been prepared by "in-situ" copolymerization of the monomers in the presence of porogenic diluents. The main factors affecting the porous structure and the loading of hydroxyl groups of the monolithic columns were investigated. In order to evaluate the mechanical stability of the column material, the pressure drop across the column was measured in different flow-rates. Using phenol, resorcinol and catechol as the test compounds, a normal-phase character of the poly(vinyl ester resin-co-ethylene dimethacrylate) monolith was evident. Second, the monolith was further derived to prepare diol column. Lysozyme and IgG were separated by the columns with a spacer arm and the other without spacer arm. Moreover, the adsorption capacity of the column has been investigated. Third, a strong cation-exchange stationary phase was synthesized and then evaluated by the separation of a-amylase and lysozyme. Fourth, the other new monolithic column for HPLC was prepared using vinyl ester resin not only as the monomer but also as the crosslinking agents. The main factors affecting the porous structure and chromatographic characterization of monolithic columns were also investigated. Finally, a molecularly imprinted polymer (MIP) has been prepared using diniconazole as the template. HPLC was used to study the molecular recognition mechanism regulating the binding behavior and evaluate the binding performance of MIPs for diniconazole and paolobutrazol. The results showed that the MIP had significant molecular imprinting effect and had high affinity and selectivity for diniconazole. Moreover, the diniconazole-imprinted polymers had the ability to enrich trace amount of diniconazole.