Studies On The Insecticidal Components Of Celastraceae Plants In Qinling Mountain Area

The study was funded by National Natural Science Fund, which was a part of theproject "Study on Lead Optimizing of Novel Insect Digest poison and Action Target" (NO.30130130). Based on the preliminary screening results of insecticidal activities among 13species Celastraceae, 11 chemicals were isolated from 3 species plants (Celastrusgemmatus Loes., C. rosthornianus Loes. and Euonymus japonicus Thunb.), and 6chemicals exhibited insecticidal activity. The process of isolation and purity of insecticidalchemicals were guided by the bioassay and phytochemistry method, such as the columnChromatography, Preparative TLC and Preparative HPLC, have been used. Six compounds (NW02, NW04, NW05, NW06, NW08, NW10) were isolated fromroot bark of Celastrus gemmatus Loes, and the structure were elucidated by spectralmethods. NW06 is benzoic acid and NW10 is β-sterol, they are both known chemicals.NW02 is 6β-diacetoxy-1α,9β-difurancarbonyloxy-β-dihydroagarofuran and NW08 is thesame as it, NW04 is 6β-diacetoxy-9β-benzoyloxy-1α-difurancarbonyloxy-β-dihydroagarofuran, NW05 is2α-acetoxy-1α,9β-dibenzoyloxy-β-dihyfroagarofuran. Based on searching on novelty,above three chemicals are all novel nature products. Two new compounds (LY01 and LY02) were isolated from root bark of C.rosthornianus Loes. LY01 is 1α-acetoxy-6β,8β-dibenzoyloxy-4β,9β-dihydroyl-2α-( α-methylbutanoyloxy ) -β-dihydroagarofuran and LY02 is1α,9β-diacetoxy-2α,8β-dibenzoyloxy-4β,6β-dihydroyl-β-dihydroagarofuran. Three compounds (LY11, LY14 and LY19) were isolated from root bark of Euonymusjaponicus Thunb.. LY11 isβ-sterol, LY14 is Celangulin V which was first reported fromCelastrus angulatus Max., LY19 is 1 α ,2 α ,4 β ,6 β ,8 β ,9α,13-hepthydroyl-β-dihydroagarofuran. Both LY14 and LY19 were isolated from the plantat the first time. The insectidical activies of six compounds with β-dihydroagarofuran sketon againstthe 3rd instars larvae of Mythimna separata Walker and Plutella xylostella were tested. Theresults showed that 5 compounds exhibited the stomach-toxicity activity, the order ofactivity is NW04> NW05> NW02>LY02> LY01, but LY19 did not exhibit activity. Themortality of NW04 against Mythimna separata Walker and Plutella xylostella were 100%and 50% at the concentration of 2.5% (W/V) respectively, 70% and 20% at theconcentration of 1.25% (W/V) respectively.