| In this study, effects of dietary protein levels on the growth of tadpole and pen rearing on morphological characteristics and product quality of northeast forest frog were investigated.In the first experiment, 10 diets with different levels and sources of proteins were fed to 10 groups of tadpoles kept in laboratory. Tadpole samples were collected and weighed every 5-day. Metamorphosis rate and duration of tadpole phase of each group were also recorded.Dietary treatments had significant effect on the growth and development of tadpoles (P<0.01).As dietary CP levels increased, tadpoles grew faster and LBW gain was increased during testing time. Maximum LBW of the tadpole, highest DMD and LBW of new metamorphosed frogs were positively and linearly related to dietary CP levels. The time of both tadpoles' phase and metamorphosis were negatively related to dietary CP levels with correlation coefficients being -0.966 and -0.892 respectively.Tadpoles were omnivorous. Their growth was related to dietary CP levels, not related to sources of dietary CP. In terms of tadpoles' growth, metamorphosis and health mark, dietary CP requirement for tadpole wasl 5%.In the second experiment, comparative analysis of morphological characteristics, body type indexes and production performance of Oviductus Ranae were carried out to detect the effect of pen rearing on forest frog. The results were showed as follows:The rate of forest frog with dark spots on the back of pen fed frogs was significantly decreased (P<0.01) compared with the wilds. The color of belly skin tended to become light in pen fed frogs. Compared with wild female frogs, the pen fed females was shorter in body length (BL), shorter in hind leg length (HLL), but bigger in body weight (BW), higher in relative fatness (RF), higher in body weight/body length index (WLI) and higher in yield of Oviductus Ranae (YOR). The correlation coefficient between BL and YOR in pen rearing female frogs was significantly(P<0.01) increased, compared with that of the wild females.The correlation coefficients between YOR and HLL, YOR and BW, YOP and WLI in pen fed female frogs did not differ (P>0.05) from those in the wild females. In addition, the correlation coefficients between BW and BL, BL and HLL in pen fed female frogs were significantly (P<0.01) increased compared with those of the wild females.In the third part of this study, the effect of pen feeding on chemical components of the OR , meat and bone meal and skin of forest frog was evaluated. Items analyzed included common shape and properties (color, fatty luster, transparency and coefficient of expansion ), common chemical components (CP, CF, cholesterol, total phosphoiipids, etc.), amino acids, trace elements, total polysaccharides, fatty acids and peptides.The color of OR differed significantly between wild and pen fed frogs. The color of wild OR was from golden-yellow to yellow white before the end of the producing year. While, the color of pen rearing OR was from yellowish pink to light yellow.There were some significant differences in chemical components between wild OR and pen rearing OR. CP of wild OR was higher than that of pen rearing OR by 5.19%. EE of pen rearing OR was 2.7 times as high as that of wild OR. The contents of total phosphoiipids and cholesterol of wild OR were higher than those in pen rearing OR by 19.5%and 25.8% respectively. Ala was not found in pen rearing OR. Lys in pen rearing OR was higher than that in wild OR by 81.2%. Both total relative content of essential amino acids and E/N (essential amino acids/non-essential amino acids) in pen rearing OR were higher than those in wild OR by 13.18% and 29.41% respectively. In pen rearing OR, the contents of Zn and Cu were 3.9and 1.6 times of those in wild OR. The content of Mn in wild OR was 14.8% higher than that in pen rearing OR. The content of total polysaccharides of wild OR was 8.78% higher than that of pen rearing OR. GC/MS was applied to study fatty acids in the two samples above. Results showed that both wild OR and pen rearing OR ma...
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