The Study On Molecular Mechanism And Physiology Of Drought-tolerance Of Wheat Changwu 134

With less drought-resistant Zhengyinl (weak droughty-resistance) as control and under the water stress at -1.0 MPa PGE 6000, the water metabolism, metabolism of osmotic regulation, the change of the anti-oxidation system as well as the relationship of the production of water stress induced protein and water regimes of Changwu 134 are studied, aiming at profoundly understanding the molecular and physiological mechanism of drought-resistant wheat Changwu 134 and providing the theoretical basis for breeding drought-resistant wheats.Results demonstrate that under water stress, Changwu134 has higher relative water content, water potential, Stomatal conductance, and transpiration rate and lower water saturation deficit than Zhengyinl. This shows that drought-resistant wheat Changwu 134 has stronger capability to resist water losses under water stress.Osmotic regulation is the important physiological mechanism for plants to adapt to drought-adversity. Under water stress at -1.0MPa, the amount of osmotic regulators of Changwu 134, such as soluble sugar, proline , K~+ are the lower at the early stage than Zhengyinl , while after a specific time of water stress, the three indexes of Changwu134 climb steadily and continuously, and finally higher than Zhengyin1. Obviously, Changwu 134 has stronger ability to regulate osmotic during continuous water stress, therefore, a more normal water metabolism is maintained in wheat body.Under water stress at-1.0MPa, the results of the study on the anti- oxidation system show that the capacity of protective enzyme system of strong drought-resistant wheat Changwu 134 increases drastically, and has higher ability to eliminate the activated oxygen from metabolism and prevent cell structure from being damaged whereas its non-enzyme system is rather stable. The capacity of protective enzyme system of less drought-resistant wheat Zhengyin 1 is weaker while the content of its anti-oxidant in non-enzyme system is higher and its overall capacity of anti-oxidation is weak. That is probably one of the main reasons why changwu 134 has stronger ability to resist drought.Under the water stress at - 1.2MPa PEG 6000, the 39.5KD water stress induced protein and 48.5KD water-sensitive protein have been induced from plumule of drought-resistant Changwul34and purified.The two proteins are transblotted onto polyinylidene difluoride (PVDF) membrane in Tris-Gly electrical transfer buffer. The N- terminal acid amino acid sequences of the proteins are analyzed in advanced method. The sequence of amino acids of water stress induced protein is determined as SKIKIGINGF, which is identified as glyceraldehydes-3-phosphate dehydrogenase from the data in GenBank.