Studies On SNPs Of Myostatin Gene And Genetic Diversity Of Some Chinese Geese Breeds(Groups)

The nucleotide acid sequences and framework characteristics of 3′-regulatory region (GenBank entry number was DQ291134) and intron 1(GenBank entry number was DQ355160) of myostatin gene in goose were first studied, and contributions of different region sequences in myostatin gene, such as cDNA, 3′-regulatory region and intron 1, to the evolution process were primary discussed based on other species'information offered by GenBank. Based on myostatin gene sequence of goose, 15 pairs of PCR-SSCP primers were designed by Oligo 6.0 and Primer Premier V5.0 which aimed to detect single nucleotide polymorphism(SNPs) of 3 geese groups. The relationships between SNPs and the slaughter characters, such as slaughter body weight, breast muscle weight, breast muscle percentage, limb muscle weight, limb muscle percentage, eviscerated carcass weight, eviscerated carcass percentage and so on, were explored. Meanwhile, six microsatellite primers were seriously selected. Allele frequencies, heterozygosities(H), polymorphism information content (PIC) and relative relationship among 8 geese breeds(or groups) including Yan goose, Wulong goose, Yangzhou goose, Zhedong goose, Xingguo goose, Siji goose, and a introducing breed-Laiyin goose, were also analyzed. The results were as follows:1. Nucleotide acid composition in 5′-regulatory region of myostatin gene showed that A+T was accounted for 63.32% and G+C 36.44%.The putative transcription initial site was identified at 117bp upon exon 1, and"CCAAT"box and"TATA"box were identified at﹣73bp and﹣27bp upon the transcription initial site respectively. Nucleotide acid homology was high to 99% when compared to the same sequence of goose logged...