Isolation, Chromosomal Localization Of Three Bovine Gene And Their Effects On Bovine Economic Traits

309 blood samples from 8 cattle breeds- Luxi, Qinchuan, Jinnan, Charolais, Angus, Limousin, Simmental and Hereford were collected to extract genomic DNA ,and some liver tissue samples from 3 cattle breeds-Angus, Simmental and Hereford were took to extract total RNA in this study.Then the isolation ,identification and sequence analysis to the bovine FABGL gene, ANGPTL4 gene and ACAS2 gene for the cDNA sequence and genomic sequence was performed by homology cloning and bioinformatics approach combined with RT-PCR, 3'and 5'RACE and electronic-PCR technique. Afterwards, the precise location of both three gene were determined by using the SUNbRH hybrid panel. And then , detection of SNPs within some genomic fragments of the three gene were employed with the use of sequencing and PCR-SSCP method. Finally, the association among FABGL and ANGPTL4 gene with some bovine growth traits and meat quality traits in 8 populations were investigated. The main results were as follows:1. Combining the In sllico cloning, RACE and RT-PCR method, the main cDNA sequence of FABGL and ANGPTL4 gene, of which contained complete CDS ,and the partial cDNA sequence of ACAS2 gene were cloned and identified. Both sequences have been submitted to GenBank database, the accession number were DQ355520,DQ355521 and DQ459834,respectively. The amino acids were also been deduced and the motifs were been predicted.The ruslts showed that the FABGL protein and ANGPTL4 protein were composed of 260 and 410 AA respectively.2.Entire 3'cDNA sequences of FABGL and ANGPTL4 gene and partial 5'cDNA sequences of both two genes were isolated , whole 9 exons and 8 introns contained in FABGL...