Detection And Identification Of Fungal Aerosol And Related Mycotoxins In Animal Raising Environment

The animal raising environment is an important source of airborne fungi and mycotoxins for natural environment. Domestic animals often suffer from many mycosis and mycotoxicosis, which are caused by airborne fungi in animal raising environment. Their universality has imposed a serious threat to the development of animal raising industry. The harm on raising animal could be reduced by controlling the level of airborne fungi and mycotoxins in animal raising environment. China has become the biggest domestic animal raising country in the world, but its literature and systemic study on airborne fungi and mycotoxins in animal raising environment are very sparse. Therefore, the systemic evaluation of airborne fungi and mycotoxin in animal raising environment, especially in chicken house, was carried out by use of the internationally standard air sampler and advanced trace mycotoxin detection technology.1.Quantification of airborne fungi in different structure animal raising environmentAir samples in different structure chicken, pig, rabbit, and cow raising environments of Shandong province, all together 12 houses, were performed by FA-1 (Andersen-6) internationally standard solid impingement air sampler and RBC agar as isolation medium. The number of CFU (Colony forming unit)was counted, the fungal genera were identified. From 126 samples (756 Petri dishes), total 7.77×103 CFU living fungal particles were captured. The concentrations of airborne fungi in sampling environment were 1.1-3.0×103 CFU/m3, the value of count median diameter (CMD) is 2.6-4.1,and the value of geometric standard deviation(GSD)is more than 1.6. The majority of airborne fungi were mainly distributed on D grade. The predominant fungi in all sampling feeding houses were identified, altogether 21 genera as follows Aspergillus, Penicillium, Alternaria, Cladosporium, Fusarium, as well as Acremonium, Bipolaris, Botrytis, Coniothyrium, Curvularia, Graphium, Mucor, Rhizopus, Myrothecium, Paecilomyces, Phoma, Rhodotorula, Saccharomycess, Scopulariopsis, Scytalidium and Trichoderma. The concentrations (1.8-3.0×103CFU/m3) of airborne fungi in close environment were obviously higher than those of semi-close...