Cryopreservation Of Germplasm Of Ornamental Ferns

China has the reputation of kingdom of ferns. There are 12,000 species of ferns all over the world, and there are more than 2,600 species among which 500~600 species are specially distributed in China, covering one fifth of the whole species of the world. Recently, due to the destroyed natural environment and unreasonable exploitation and collection, some rare species are endangered. Thus it is urgent to strengthen its correlative fundamental research, propagation and conservation technology, which is very important to conserve and utilize the germplasm reasonably. Based on the research on the spore sterile culture and gametophyte development, it was taken five years to investigate the cryopreservation procedure of fern spores, gametophytes and green globular bodies. In addition, seedlings of Arabidopsis thaliana were selected as plant material; the effects of heat stable proteins expression arose by the solution treatment in cryopreservation by vitrification were carried out and to guide the amendment of plant vitrification solution. The results were summarized as follows:1) Protocols have been developed for the spore sterile culture of Alsophila gigantea var. gigantea Wall. ex Hook., Pteris cretica'Albo-lineata', Pteris vittata L. and Cyrtomium fortunei J. Smith.2) The spores of sixteen species of ferns were cultured in the inorganic medium and the soil respectively. The process of spore germination and the prothallial development were observed and recorded by microscope. The nature of spore germination, formation of thallus in the each development stage and time needed to reach every stage were recorded. These results offered important basis for the studies on the evolutionary process...