Study On Semiochemicals Of Dioryctria Pryeri

Dioryctria pryeri is one of the most destructive pests of conifer,distributed in Heilongjiang,Liaoning,Jilin,Neimenggu,Shandong,Hebei,Beijing,Henan,Shanxi,Gansu, Qinghai,Xinjang,Anhui,Jiangsu,Zhejiang,Sichuan,and Taiwan Provnce in China,and Japan, whose distribution matches to the the distributiong of host plants.Recent years,Outbreaks of Dioryctria pryeri have caused great damages to economy and ecological envioronment in Xing'an Mountins,Lushuihe,Huangnihe,Erdanbai of Changbai Mountain areas.The paper began with its biological and ecological characteristic.Host volatiles of semiochemicals were studied,in order to analyse of volatiles from cones on different damage periods,volatiles from different tissues on overwinter larvae transfer damage period,and time variational rule on adults oviposition period,larvae forage different tissues of Pinus koraiesis characteristic and tested active components of volatiles.Soluble surgar and total nutrigen contents of cones were analysd,so as to clarify the relationship between nutrition substance contents and damage of larvae.Sensillum of adult antenna,larvae antenna and mouthparts were observed by scanning electron microscope,which supply data to larvae forage host selection,and chemical communication.All of them will establish basis of control Dioryctria pryeri without social effects of pollution.Observe the biological characteristic with culture in laboratory and observation in fields. Adopted diagonal and random sample in fields,and aggregate index,regression analysis to analyse spatial distribution of ecological characteristic,found that the larvae distribution in fields,different positions and layers of tree crown,and different parts of cones is aggregation.Volatiles from host were collected by Headspace Sample,components and relative contents were analysed by TCT-GC/MS.Olfactory response were used to test larvae esponse to different tissues on larvae transfer damage period,to find that larvae reponse standard sample compounds of volatiles from cones to alcohol,while reponse sigle component a-pinene,Limonene,and double components a-pinene+β-pinene to standard sample compounds of volatiles from cones,which can draw the conclusion that single component substance play a key role in larvae host selection on overwinter larvae transfer damage period.Terpenes from healthy and damaged cones varied on adult oviposition period;there is significant difference between contents of most volatiles in pm,significant difference between am and pm,no significant difference in am.a-pinene,β-pinene and limnene has the most contents and same change of volatiles from healthy and damaged cones on different days.Voltile components released from cones on overwinter larvae transfer damage period are more than that on adult oviposition period. Olfactory basin and Y tube olfactory were used to test larvae response to Pinus koraiesis different kinds of cones,find that those response damage cones to healthy cones in mixture and plant forests.That response to different kinds of damaged cones which form from the different parts of cones damaged by Dioryctria pryeri,is Pinus koraiesis cones above damaged＞cones below damaged＞cones middle damged＞cones total damaged in mixture forests, while is Pinus koraiesis cones total damage＞cones below damaged＞cones above damaged＞cones middle damaged in plant forests.Anthranone method and Kjeldahl Determination were adopted to test soluble surgar and notrigen contents of Pinus koraiesis cones,cone axis, cone scale,find that there is tight correlation between larvae forage apt and soluble surgar,and related with the number of eat hole and quality of Pinus koraiesis cones.Adopting Anthranone method and Kjeldahl Determination test soluble surgar and total nutrigen contents of cones on different damage periods.The content of soluble surgar is lower than nutrigen on larvae transfer damage period and adult oviposition period,which is revised on transfer overwinter period,which is the same in cone axis and cone scale.In addition,the content of soluble surgar is increased and total nitrogen is decreased with cones growth.And, soluble content of healthy cones is lower than that of damaged cones,which is revised of the total notrigen content,which was the recovery strategy of cones to larvae forage.Larvae forage cone axis,soluble conent is that cones＞cone scale＞cone axis,and total nutrigen is cones＞cone axis＞cone scale.Scanning electron microscope was used to observe sensillum of adult antenna and larvae mouthparts and antenna.Sensillum trichoidea,sensillum chaetica,sensillum auricillica and sensillum coeloconica distributed on adult antenna,and sensillum trichoidea,sensillum chaetica,sensillum coeloconicais are chemical sensillum which recept sex pheromone, mechanical stimulate,and water vapor,nutrigen dioxide,humidity and odor of plants, respectively.There is no sensillum on antenna and mouthparts of the fifth age larvae,while there are 2 sensillum styloconica,2 sensillum basiconica and 1 sensillum trichoidea on the fourth age larvae attenna terminal that recept ordor,sensillum chaetica on the second antenna that recept ordor,2 sensillum styloconicaon on inner side of outer galea and 4 sensillum styloconicaon on terminal of palpus maxillaris that recept odor nutrition component when forage,1 sensillum styloconica on palpus labialis is longer than sensillum basiconica on palpus labialis.