| PCR-SSCP method was used to study the SNP of OPN and CAM genes that might be correlated with reproduction traits and egg quality traits in Jinghai Yellow chicken, and the systematic investigation was conducted on Jinghai Yellow chicken OPN and CAM genes to evaluate the association of molecular markers with growth traits, reproduction traits, egg shell quality traits, carcass traits and meat quality traits.The main results are listed as follows:1. The full length DNA of OPN gene was obtained using the techniques of PCR-SSCP. 20 OPN SNPs were found in Jinghai Yelow chicken:A306T,A459C,G1367T,C1389G,G1399A,A1443G,T1463C,A1489G,G2219C, C2225T,G2257C,G2283C,A2288G , G3010A , G3159A , G3770A , C3830T , A3887G , A3918G, 3217T deletion/insertion。2. Five CAM SNPs were found in Jinghai Yellow chicken: G56A , A57G and C96T(GenBank NO: M31605);T479C and G480A(GenBank NO: L00101)。3. At the OP2 locus of OPN gene, birds with h1h1 genotype has the lowest value in egg shell index compared with h1h2 genotype (P<0.05). Birds with h2h2 genotype have significant higher value in semi-eviscerated weight ratio and eviscerated weight ratio than with h1h1 genotype in male birds, and birds with h1h2 has higher value in breast meat ratio than birds with h2h2 genotype (P<0.05). The contribute percentage (CP) of water holding capacity (WHC) in thigh meat with h1h1 genotype was -15.41%, and with h1h2 genotype was 11.82% in males. The contribute percentage of shear force in thigh meat with h1h2 genotype was 9.41%, and the CP with h2h2 genotype was -22.78% in females. The main effete index (MEI) of semi-eviscerated weight ratio, eviscerated weight ratio, breast meat ratio and WHC in thigh meat was all over 10% in males, and the MEI of shear force in thigh meat was 12.46.4. Significant differences were found between genotypes in OP5 locus and semi-eviscerated weight ratio, eviscerated weight ratio, breast meat weight and thigh meat weight in females. CP of GG and GT genotype were high of shear force in breast meat, and the values were 25.97% and -15.06% in females. The (MEI) of semi-eviscerated weight ratio, eviscerated weight ratio, breast meat ratio, thigh meat weight and shear force in thigh meat were 13.25%, 15.54, 13.91, 18.42 and 16.03 in females.5. Significant differences were found between genotypes in OP6 locus and age at first egg and egg numbers at 300 days. CP of h2h2 genotype was -9.26% of egg numbers at 300 day birds. The MEI of breast meat weight was over 11 in both sexes.6. At the OP2 locus of OPN gene, CP of h2h5 genotype was -8.83% of egg shell strength. The MEI of abdominal fat ratio, breast meat ratio, shear force in breast, WHC in breast meat and thigh meat were 18.10, 9.28, 16.37, 20.41 and 29.39 in females.7. Significant differences were found between genotypes in OP12 locus and growth traits: h1h6 genotype was the latest predominant genotype that increased body weight. The MEI of live weigh, and thigh meat weight were over 11% in both sexes. For abdominal fat ratio, CP of h3h3 genotype was -19.72% in females.8. Significant differences were found between genotypes in OP15 locus and growth traits: h5h5 genotype was the one lest predominant genotype that increased body weight, h1h2 genotype was the predominant genotype that increased body weight, but h1h2 genotype was the one lest predominant genotype that increased egg numbers. For calcium weight in egg shell and calcium ratio in egg shell, CP of h2h4 genotype was -14.61% and -13.67%. Significant differences were found between genotypes in OP15 locus and abdominal fat ratio: birds with h2h2 genotype have significant high value than with h2h2 genotype, and MEI of abdominal fat ratio was 12.44 in females.9. At the CA2 locus of CAM gene, significant differences were found between genotypes in and egg numbers at 300 days and egg numbers at 66 weeks: h2h2 genotype was the latest predominant genotype that increased egg numbers. Egg strength of h3h3 genotype has the lowest value than with other genotypes, and the CP was -24.69%. The MEI of live weigh, and thigh meat weight were over in both sexes. For abdominal fat ratio, CP of h3h3 genotype was -19.72% in females. CP of shear force in breast meat of h1h3 genotype was -19.97% and 45.05% in males and females respectively, and MEI of shear force in breast meat was 21.35 and 33.24 in males and females.10. At the CA5 locus of CAM gene, CP of abdominal fat ratio of AA and BB genotype was 32.50% and -15.03% respectively in females.11. Significant or very significant interaction effect of OPN and CAM were found on birth weight, body weight at 8 week, body weight at 12 week, egg shell weight, egg shell strength, egg shell index, egg shell percentage, grown up weight, eggs at 300 day and eggs at 66 week in Jinghai Yellow chicken...
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