Syndrome Characteristics Of Rheumatoid Arthritis And Its Correlation With Inflammatory Cytokines

ObjectiveCurrently, the Rheumatoid arthritis syndrome type in Mongolian is no uniform standards, to a certain extent, affected the Mongolian medicine for RA Diagnosis and treatment. The experiments is accordance with the requirements of clinical epidemiology, through the180cases of patients with RA-looking Mongolian syndrome and objective study of feature, in order for the standardization of Mongolian medicine clinic RA, RA Mongolian syndromes is established standards provide the basis for the development of treatment programs. By observing the Mongolian syndromeand serum TNF-α, HIF-1α, VEGF-A correlation of experimental basis from an objective study of the micro-level Mongolian syndromes, with some innovation, promote Mongolian medicine modernization of the research process.MethodsIn this study, cross-sectional and prospective research methods to collect180cases met the diagnostic criteria for RA patients. Surveying RA patient general condition, Includingmedical history, symptoms, tongue and pulse condition, urinate, cabinet, Record routine testing laboratory indicators (RF,CRP, CCP antibody,GPI.AKA, APF, IgA, IgG, IgM),While using double antibody sandwich enzyme-linked immunosorbent assay (ELISA) to detect180cases of RA patients and30healthy human serum TNF-α, HIF-1α, VEGF-A levels. The traditional Mongolian disease diagnosis "Three clinics" combines modern mathematical statistical methods, explore the distribution of RA Mongolian syndrome. Logistic regression analysis of multi-element method, further adoption of Mongolian syndrome factors for RA were analyzed to180cases in patients with RA TNF-α correlation analysis HIF-1α, VEGF-A and RA Mongolian different syndromes and laboratory routine testing between indicators. Statistical analysis was performed using SPSS19.0software package. RA clinical data were analyzed using descriptive frequency and relative frequency analysis, measurement data using two independent samples nonparametric rank sum test; level data using rank sum test; count data×2test and rank sum test was used to compare between the two groups rank sum test. Mongolian syndrome were analyzed using non-conditional Logistic regression analysis. Bian correlation between serum inflammatory cytokines and laboratory parameters related to analytical methods used Spearman.Results1General conditions and duration: RA Mongolian medicine Hari Taolie, Chagan Taolie, Alaga Taolie three syndrome types in Hari Taolie proportion is highest, Alaga Taolie minimum. The proportion of women and men nearly5.21:1. Patients between the ages of30to60years old, A total of137cases, Accounted for76.1%, Hari Taolie age is small, Chagan Taolie older.1-5years of disease duration, Chagan Taolie long course of disease, In course of the disease in patients with more than5years, Hari Taolie short course of disease, Found in course of the disease in patients within five years, Alaga Taolie found in the course of each stage.2Correlation with different syndromes Mongolia RA clinical symptoms and laboratory tests between indicators:Mongolian joint pain points between different syndromes, joint tenderness integration, joint swelling points, the total number of swollen joints, CRP, GPI comparison Hari Tao Tao Tao Lie Chagan, A La Ga Tao Lie differences were statistically significant (P<0.05), check out the A La Ga Tao Lie comparison between A La Ga Tao Lie no significant difference (P>0.05). Different syndromes joint dysfunction points compared with Hari Chagan Tao Tao column column, A La Ga pottery out the difference was statistically significant (P<0.05), Hari Tao Lie A La comparison between Ga Tao no statistical difference significance (P>0.05). Among the three groups Mongolian syndromes, RF were significant differences (P<0.05), sort of Hari> A La Ga> Chagan.Duration of morning stiffness Chagan Tao Lie is the longest, IgG, IgM highest average, but among the three groups, the difference was not statistically significant (P>0.05). A La Ga pottery anti-CCP antibody column, IgA mean and AKA, APF constitute positive than the maximum, but the difference was not statistically significant (P>0.05) among the three groups Mongolian syndromes.3RA Mongolia syndromes:RA signs and symptoms appear Mongolia frequency of pain has given place, rainy days increase, small smell of urine, chills, warm, cold joints, limbs not warm, pain, pale tongue, Yaoxisuanruan, tired, thin and greasy yellow tongue, red tongue, flexion and extension negative, joints red, thirst, joints, fever, joint pain, slippery pulse, rapid pulse, urine smell great pulse, burning, heat intensified pain very night, wet, tired thin white fur, urine yellow, dry stool, clock jitter, body heat and so on. Pain has given place, rainy days increase up to75%of patients, chills, warm, cold joints, limbs not warm more than50%of patients, joint redness, thirst, joint fever more than40%of patients.180cases of RA patients with symptoms and signs of Mongolian non-conditional Logistic regression analysis, and the use of Forward LR method for analysis of the results obtained, factors related to Mongolian Syndromes section swelling, joint pain, rapid pulse, yellow fur thin greasy, urine smell great, flexion and extension negative, tired, weak pulse, low urine odors, numbness, itching, clock jitter, pale tongue and so on. Hari pottery which factors out the relevant section of swelling, joint pain, rapid pulse, thin greasy yellow fur, urine odor and large heat symptoms and signs; influencing factors Tsagaan pottery column joint pain, flexion and extension negative, tiredness, pulse weak, pale tongue, urine odor and other cold symptoms and signs; influencing factors A La Ga Tao Lie about pottery festival swelling, joint pain, numbness, itching, clock jitter, pale tongue and other symptoms and signs of cold and heat. Logistic regression model to predict the correct total Hari Taolie rate reached88.3%; Chagan Taolie reached94.4%; A La Ga Tao Lie to89.4%4Correlation of serum inflammatory cytokines in RA patients and between laboratory indicators:180RA patients and30healthy age, gender ratio was not statistically significant (P>0.05), comparable. Between diseased and healthy human serum TNF-α, compare HIF-1α, VEGF-A levels were significantly different (P<0.05). Mongolian TNF-α, HIF-1α, VEGF-A levels between the different syndromes comparison: Hari Tao LieTNF-a highest level, with Chagan Tao Lie there was a significant difference (P<0.05), and A La Ga Tao there was no Lie significant difference (P>0.05), Chagan Tao Lie with a La Ga Tao Lie there was a significant difference (P<0.05); highest Hari Tao LieVEGF-A, HIF-1α levels, and Chagan Tao Lie, A La Ga Tao Lie There were significant differences (P<0.05), Chagan between Tao Lie and a La Ga Tao Lie was no significant difference (P>0.05). Analysis of correlation between TNF-α, HIF-1α, VEGF-A in serum of RA patients and laboratory parameters showed TNF-α and RF, CRP was positively correlated,VEGF-A and RF, HIF-1α,TNF-α were positively correlated, HIF-1α and TNF-αwere positively correlated.Conclusions1RA early Mongolian syndromes to Hari Tao Lie, followed by A La Ga Tao Lie, Chagan Tao Lie. RA in late to Tsagaan Tao Lie and A La Ga Tao Lie is common, A La Ga Tao Lie rare.2Joint pain points, joint tenderness integration, joint swelling points, the total number of swollen joints and CRP, GPI, RF ceramic column as Hari objective type basis. Joint dysfunction points can be used as an objective of Chagan Taol type basis. This study confirms the Mongolian RA dialectical theory of governance is rich material base and provide a scientific basis for the feature of RA Mongolia and Mongolia syndrome dialectical type of objective research.3This study confirms the traditional Mongolian medicine "three clinics" dialectical approach to RA diagnosis typing rate is relatively high and should be introduced Mongolian traditional "three clinics" approach, and laid the foundation for further study of RA Mongolian Syndromes.4RA patients with serum TNF-α, HIF-1α, VEGF-A levels were significantly higher, indicating that serum TNF-a, HIF-1α, VEGF-A determination can be used as indicators of joint detection diagnosis of RA, RA between different syndromes TNF-Mongolian a, HIF-1α, there was a significant difference in VEGF-A levels. Show that there are differences RA Mongolian syndromes pathological mechanisms at the molecular level, Haritaoli TNF-α, HIF-1α, VEGF-A level are the highest,and inflammatory cytokines in patients with RA and RA activity indicators are intrinsically linked, from the micro level to provide an objective basis for the treatment of RA Mongolian Syndromes.