Experimental Study On The Immunological Characteristics Of Peripheral Blood T Lymphocytes In Patients With Sjogren 's Syndrome

Objective:To study on in immunological characteristics of T lymphocyte in peripheral blood from patients with Sjogren’s syndrome (SS), provide the theory basis for the further research of Traditional Chinese medicine (TCM)targeted therapy.Methods:T lymphocyte subsets in peripheral blood from SS,RA and normal control groups were tested with various monoclonal antibodies and multicolor flow cytometry. T lymphocyte subsets were tested in peripheral blood from SS patients of different TCM Syndromes.The relationship between T lymphocyte subsets and disease activity was measured by erythrocyte sedimentation rate (SR)and serum IgG.Results:1.The percentage of CD4+T cells and the ration of CD4+/CD8+T cells in peripheral blood from SS patients decreased significantly, and the percentage of CD8+T cells increased than that in control groups (P<0.05).2.Compared with patients in inactive period, the percentage of CD4+T cells in peripheral blood from SS patients in the active stage increased (P>0.05), the percentage of CD8+T cells decreased(P>0.05), and the ration of CD4+/CD8+T cells increased(P<0.05).3.Compared with control groups, the percentage of CD69expressed on T cells in peripheral blood from SS patients decreased,and the percentage of CD95’s expression increased (O<0.05). The percentage of CD69CD95’s expression also increased (P>0.05). 4. Comparison of SS group:compared with patients in the inactive period, the percentage of CD69expressed on T cells in peripheral blood from SS patients in the active period increased, the percentage of CD95and CD69CD95’s expression decreased (P>0.05).5.There were no differences among CD4+T cells, CD8+T cells and CD4+/CD8+in peripheral blood from SS patients of different TCM Syndromes(P>0.05).6. CD69and CD95’s expression on SS patients of different TCM Syndromes was no significantly different(P>0.05).7.T cell receptor Vβ(TCR Vβ) subfamilies gene in peripheral blood from SS patients and control groups were expressed in all. Compared with control groups, Vβ5.3, Vβ13.1, Vβ12, Vβ7.2had significant differences (P<0.05).8.Comparison of different TCM Syndromes in SS group:Vβ13.1and Vβ14were significantly different (P<0.05).Conclusion:There was dysimmunity on T lymphocyte subsets in peripheral blood of SS patients, which was related to disease activity; apoptosis after activation of autoreactive T cell may be induced by Chinese medicine; TCR Vβ subfamilies of different TCM syndromes were different, which noted that different autoreactive T cells of abnormal activation tended to result in different expression of TCM Different syndromes.