The Study Of T-lymphocyte Subsets' Adjustment By Ozone Therapy In Patients With NHL

Objective:The present study is designed to contrast the different clinical effects of the T-lymphocyte subsets' adjustment in peripheral blood of patients with Non-Hodgkin's lymphoma between the methods of the Ozone therapy and normal chemotherapy,and to provide a new way to treat lymphoma.Methods:There are 67 patients with Non-Hodgkin's lymphoma being derided into two groups randomly.Treating group of 37 patients is given Ozone therapy during the chemotherapy, combined with major O₃ auto-haemoyherapy and rectal O₃ gas insuffiation.Contrasting group of 30 patients is given the same chemotherapy separately.Each is given two cycles.The quantities of CD3⁺,CD4⁺,CD8⁺ in peripheral blood,the ratio of CD4⁺/CD8⁺,and IFN-r and IL-4's expression in CD4⁺T lymphocytes are detected by FCM to reflect the quantities of Th1,Th2,the ratio of Th1/Th2.At the same time evaluate the clinical effect,and change of tumor-makers: LDH andβ₂-MG.Result:(1)Compared with the normal controls peripheral CD3⁺,CD4⁺,Th1 cell and CD4⁺/CD8⁺,Th1/Th2 ratio reduce,while the CD8⁺ cell increased,Th2 cell has no obvious changes in patients of NHL.(2)After treatment,CD4⁺,Th1 cell and CD4⁺/CD8⁺,Th1/Th2 ratio have increased markedly,CD8⁺ cell have lowered obviously,CD3⁺,Th2 cell have no obvious changes;LDH,β₂—MG have lowered in treating group.(3) After treatment,CD3⁺,CD4⁺,CD8⁺ cell,CD4⁺/CD8⁺ ratio have no obvious changes;Th1 cell,Th1/Th2 ratio have lowered, Th2 cell has increased;LDH has lowered,β₂—MG.has no significent changes in contrasting group.(4) After treatment,treating group's CD4⁺,Th1 cell,CD4⁺/CD8⁺,Th1/Th2 ratio are higher than contrasting group's;while CD8⁺,Th2 cell,LDH andβ₂-MG of treating group are lower than those of contrasting group's;CD3⁺ has no significent changes.(5) The treating group's complete response(CR) is 64.9%.Partial response(PR) is 29.7%and CR+PR is 94.6%. The contracting group's CR is 40.0%and CR+PR is 70.0%.Compare with the two groups, treating group's CR and CR+PR have significantly increased.Have the statistical significance (P＜0.05).Conclution:The initial patients of NHL have immune suppression in their bodies,we can see this point from the imbalance of T-lymphocyte subsets CD4⁺/CD8⁺,and the differentiation from lymphocyte CD4⁺ T to Th1 has reduced obviously,the imbalance of Th1/Th2 shift to Th2 side.After the chemotherapy the imbalance ratios of CD4⁺/CD8⁺,Th1/Th2 have increased.From this point we can see that the immune function of the patients of NHL has reduced much further. After combined O₃ in the treatment,the imbalance ratios have reduced.O₃ improves the immune funtion of the patients of NHL.After the Ozone therapy,the lymphoma tumor-makers:LDH,β₂ -MG have reduced obviously,this is a negative correlation of the improvement of the immune function;CR and CR+PR have improved significently,this is a positive correlation of the improvement of the immune function.Compare Ozone therapy with normal chemotherapy,it can improve the patient's immune function and clinical effects of the NHL.