Study On The Correlation Of "Taste - Pharmacodynamics - Chemical Connotation" Of Modified Ginseng

The purpose of research:Based on literature review and experiments, the study aims to explore the relationships between the flavor and property of herbs, the effectiveness of treatment and the chemical theories involved in the Adjusted Ginseng and Mume Concoction. It is hoped that the thesis could enrich the theories about’sour-sweet herbs nourishing yin’, and help improve the medical treatment of infantile diarrhea.Methodology:(1) Literature review:Drawing on traditional studies in Ming and Qing Dynasties, the study first analyses the property of each herb in the Ginseng and Mume Concoction. Based on the analysis, it explores how the concerted application of sweet and sour herbs could affect the effectiveness of Ginseng and Mume Concoction in treating children who suffer from diarrhea and have the syndrome of deficiency of both qi and yin. It also analyses how the adjustment of these herbs concurs with the principle of ’sour-sweet herbs nourishing yin’.(2) Animal experiments and Chemical Analysis:by using young KM mice as models of deficiency of both qi and yin, anminal experiments were conducted to test whether adjusting the Cinseng and Mume Concoction could generate the expected outcomes under the principle of’sour-sweet herbs nourishing yin’. Moreover, by using TLC and HPLC fingerprint chromatogram analyses, the study also examines the chemical theories underlying the concerted application of herbs in the Adjusted Ginseng and Mume Concoction. Results: (1) By adjusting the concerted application of sweet and sour herbs in the Ginseng and Mume Concoction, the rationale of’sour-sweet herbs nourishing yin’ can be proved, and the purpose of benefiting qi, promoting production of fluid, and stopping diarrhea can be achieved. (2) According to experiments, the original Ginseng and Mume Concoction can significantly increase the mice’s weight (p<0.01); the original Concoction without pungent herbs can also increase the mice’s weight (p<0.05); the original Concoction can significantly reduce the loose stools rate of mice (p<0.01); the orgininal Concoction without pungent herbs can also reduce the loose stools rate of mice (p<0.05). The original Concoction can very effectively stop the decline in the serum levels of Na+,CI-(p<0.01) and reverse the increase of serum lactic acid (p<0.01); The original Concoction without sweet, pungent or sour herbs can also effectively stop the decline in the serum levels of Na+(p＜0.01) and reverse the increase of serum lactic acid (p<0.01); The orginal Concoction and the original Concoction without sweet, pungent or sour herbs can effectively reduce the level of serum MDA (p<0.05 or p＜0.01). The original Concoction and the original Concoction without pungnent herbs can significantly increase the level of Serum D xylose (p<0.01). The original Concoction without sweet or sour herbs cannot deliver the same result.The petroleum ether extract and ethyl acetate extract in Enriched Ginseng and Mume Concortion can increase the weight of mice models (p<0.05 or p＜0.01). The two can significantly reduce the number of mice models with loose stools and effectively stop the decline in the serum levels of Na+(p<0.01); Butanol ingredient extract can also reduce the number of mice models with loose stools and stop the decline in the serum levels of Na+(p<0.05). However, none of them are effective at reducing loose stools rate. Dichloromethane extract can increase the loose stool rate of mice models (p<0.05), contrary to the purpose of treatment. The petroleum ether extract, ethyl acetate extract and N-butanol extract can modertate the increase of serum lactate (p<0.05 or p<0.01), significantly increase the level of serum MDA and reduce the level of serum D-xylose (p<0.01). Dichloromethane extract is not effective in this regard.Based on the thin-layer identification of Ginsen Mume Concoction and the HPLC fingerprint of it and its adjusted models, the study confirms that petroleum ether extract best match the peak of Ginseng Mume Concoction.Conclusion:Petroleum ether extract, ingredient ethyl acetate extract and n-butanol extract ingredients are the chemicals that make the Ginseng Mume Concoction effective at treating infantile diarrhea. They also constitute the chemical basis of the ’sour-sweet herbs nourishing yin’principle. Out of them petroleum ether extract is most important, whereas dichloromethane is irrelevant.