Studies On The Effective Constituents And Quality Of Atractylodes Macrocephala Koidz

The ethanol extract from the rhizoma Atractylodis macrocephalae was separated by column chromatography. Twelve compounds were isolated and were identified by spectra as biatractylenolide, atractylone, atractylenolide I, atractylenolide II, atractylenolide Ill, taraxeryl acetate , aniyrin acetate, atractylenolactam, junipercomphor3 P -sitosterol, i?-spinasterol, and uridine. The biatractylenolide is a new compound. The mechanism of processing was researched. Chemical component conversion during process was explained. Sesquiterpenes from Atracylodes macrocephala were researched. Atractylone can be oxidized to atractylenolide I and Ill in air: atractylenolide III can be converted to atractylenolide II in hydrochloride-ethanol. The contents of atractylenolide I and Ill both in raw and fried Atractylodes macrocephala were determined. The contents of atractylenolide I are noticeably increase after fried. The contents of atractylenolide III are different depend on the fry extent. The effects on starch enzyme activity of water extract, ethanol extract, essential oil, atractylenolide I and Lii from Atractylodes macrocephala were investigated by colorimetric analysis. Water extract can obviously increase the starch enzyme activity. Ethanol extract and atractylenolide I can also increase the enzyme activity. Oppositely, essential oil and atractylenolide III can inhibit the enzyme activity. The component's effect on absorption of small intestine to nutritional materials was also investigated by reversed small intestine. Atractylenolide I can promote the absorption of glucose and Vitamin B6 in small intestine. 7 Atractylenolidelll didn't show the activity apparently. Atractylenolide I can inhibit the spasm of rabbit intestine induced by acetylcholine. The amount-effect curve indicates the character of non- competitive. The decrease index pD2?~5.l4. AtractylenolideLll showed no activity. The pharmacokinetic character of atractylenolide I was investigated in rabbits. The contents of atractylenolide I in biological materials were determined by high-performance liquid chromatography. Plasma disposition followed a two-compartment model for atractylenolide 1 after an i.v. dose 2.5?10mg kg? Unchanged atractylenolide I rarely excreted via urine, feces and bile. Atractylenolide I was absorbed and eliminated rapidly. It distributed widely in brain, heart, lung, bile, kidney, small intestine and muscle .The protein binding was found to be high. Ethanol-water extracts from the rhizome of Atraciylodes macrocephala. Atractylodes lancea and Atractylodes chinensis were analyzed by high- performance liquid chromatography. Characteristic components distinguish Atractylodes macrocephala from Atractylodes lancea and Atractylodes chinensis were discovered. Atractylenolide I and atractylenolide 1171 were found in Atractylodes macrocephala, but not exist evidently in Atractylodes Iancea and Atractylodes chinensis. The qualitative controlling methods were studied by both thin-layer chromatography and high-performance liquid chromatography, with the atractylenolide I and atractylenolide 111 as the check samples. Both of the methods were available to qualitative analyses of Atractylodes macrocephala. The thin-layer chromatography method is selected because it is simpler than high-performance li...