Effects And Its Mechanism Of EBSPTR Therapy On Therapeutic Angiogenesis In Rats Hind Limb Ischemic Models

Objective: 1. To investigate the process of fountain and development about therapeutic Rules of EBSPTR (Eliminate blood stasis and promote tissue regeneration) in TCM and its clinical application method, so as to clarify inside of the theories and to expand its the new application area., 2.To study the efficiency and its mechanism of Shenghua soup (SHT) as remedy of The EBSPTR therapy for therapeutic angiogenesis in the ischemic disease.Methods: 1. The theories research adopts method towards bibliographic retrieval. 2. The experiment study: 50 Wistar rats (male 200-250g) were randomly divided into 5 groups: group A, normal control; group B, hind limb ischemic models control; group C, small dosage of SHT; group D, middle dosage of SHT; group E, high dosage of SHT. Rats hind limb ischemic models caused by the right side femoral artery excision. On post operation days 30 took skeletal muscle tissues samples from adductor magnus musle, which was use to assay the micro vascular density (MVD) of skeletal muscle in rats. Expression of VEGF mRNA and its receptor2 flk-1/KDR mRNA of skeletal muscle in rats was detected with ISHH methods, Expression of CD34 and was studied with immunohistochemistry.Results: 1.Therapeutic Rules of Eliminate Blood Stasis and Promote Tissue Regeneration (EBSPTR) were originated from philosophic thoughts of antiquity and the theories of Yin-Yang. The rules were developed and formed in The Ming Dynasty and The Ching Dynasty. 2.The EBSPTR have a function promote collateral vessel. The theory of EBSPTR is based on the ancientphilosophic viewpoint "things turn into their opposites when they reach the extreme." And the intermingling and combination of Yin and Vang can produce a new thing. 3.Different dosage of SHT could increase and induced significantly the expression of VEGFmRNA in rats hind limb ischemia models (p<0.05). 4.Middle dosage of SHT could significantly increase and induced the expression of VEGFR2 (FLK-1/KDR) mRNA in rats hind limb ischemia models (p<0.05). 5.High and middle dosage of SHT could significantly increased MYD of skeletal muscle tissues in rats hind limb ischemia models. (P<0.05).Conclusions: In the light of the above studies, we may safely conclude that it is reasonable and useful for rule and remedy of EBSPTR to use for therapeutic angiogenesis; EBSPTR therapy with SHT could be applicable to ischemic and occlusive vascular disease. SHT could enhance angiogenesis of ischemic tissues in adult rats. Its Action may be realized by the mechanism that up-regulation Expression of VEGF mRNA and its receptor2 flk-1/KDR mRNA of skeletal muscle in rats hind limb ischemia.