The Study On Expression Of VEGF, Receptor Tie And Angiopoietins Through Hypoxic Induction In Endothelial Cells Of Human Cerebral Arteriovenous Malformation

PART ONE: Expression of angiogenic factors and c-myc as well as its relation to proliferating activity of endothelial cells in humancerebral arteriovenous malformations[Abstract] Objective To investigate the expression of angiogenic factors and proto-oncogene c-myc and its relation to proliferating activity of endothelial cells in cerebral arteriovenous malformations(cAVM). Methods The expression of vascular endothelial growth factor(VEGF),receptor Tie, angiopoietins, c-myc and proliferating cell nuclear antigen(PCNA) was observed in 47 cAVMs of different Spetzler-Martin grades with immunohistochemistry. Results There was no significant expression of Tiel and Angl in cAVM endothelial cells, while VEGF,Tie2,Ang2, PCNA and c-myc were overexpressed when compared with fetal and normal brain blood vessels.They increased correspondingly with the grade of the cAVMs. The ovetexpression of VEGF had obvious correlation with that of c-myc and PCNA. Conclusion The overexpression of angiogenic factors might play an important role in the pathogenesis and development of cAVM, through increasing the level of proto-oncogene c-myc protein in endothelial cells.