Clinical And Experiment Research Of Reinforcing Kidney, Strengthening Qi, Nourishing Blood Therapeutic Principle In Treat Chronic Functional Of Constipation(CFC)

AIM: To observe the therapeutic effects and safety of "WenBuRunXiaFang", which based on therapeutic principle of reinforcing kidney; strengthening Qi, nourishing blood and lubricating bowels to purgation, for treatment of CFC, specially for older age of CFC Ihrough clinical and experimental study, screen out the best dose-matched manner of all the drugs in "WenBuRunXiaFang" , and therefore provided scientific evident for "WenBuRunXiaFang" in clinical treatment of CFC.METHODS1 Clinical research method: CFC examples were randomly divided in to two group: treat group (treated with WenBu RunXiaFang )and control group(treated with "BianBiTong oral solution"), aiming to observe the purgation effect and symptom improvement effect of "WenBuRunXiaFang" on treatment of CFC, and compare the curative effect of two groups and find out its side effect2 Experiment research method: Normal mice ,constipation mice and DC constipation mice randomly divided into groups, separate given orally administrated with "WenBuRunXiaFang" decoction in different dosage(30> 15 > 7.5g/kg), "BianBiTong oral solution" and distilled water respectively. Aiming to observe the purgation effect of "WenBuR.unXiaFang" on those mice. Furthermore ,used uniform design method and regression analysis method, aiming screened out41 WenBuRunXiaFang" the best dose-matched manner of all the drugs.RESULTS1 Clinical research resuhs: The results showed that there were not distinct difierence between the two groups (P>0.05), However, as analyzed the patients in different age stages, the effect of treat group was obviously better than that of control group in the patients older than fifty years. The prime symptoms of two groups after treatment were both vastly improved(P<0.05), and treat group was superior than control group in easeing defecation efforts(P<0.05)=2 Experiment research results: results showed that 'WenBuRunXiaFang'' could made the first time of passing black stools of normal mice ,constipation mice shorter: the total number of excrement drop, the total number of black stools, and the weight of excrement increased; could made total number of dry and wet faeces of DC constipation mice increased too. The Experiment results was also suggested "WenBuRunXiaFang" has the function of advancing small intestinal movement, and appears a dose-dependent manner. Combination study results indicated that four dru^Cistanche salsa , Astragalus membranaceus , Paeonia lactiflora Pall and Polygonum multiflonan Thunb ) of "WenBuRunXiaFang" play a major role in purgation action., Furthermore, among the four drugs, Cistanche salsa takes the most important role, which considered as the principle of "WenBuRunXiaFang". Astragalus membranaceus , Paeonia lactiflora Pall and Polygonum mdtiflojian Thunb ,then ,are thinked as the associate , adjuvant and messenger of the prescription respectively. Areca cathecu and Raphamis sativus exert little effect The study also screened out the best dose-matched manner of all the drugs : Cistanche salsa 25g, Astragalus membranaceus 20g. Paeonia lactiflora Pall 15g and Polygonum multiflorum Thunb lOg. Areca cathecu and Raphamis sativus were excludedConclusion "WenBuRunXiaFang" has distinctive purgation effect, clinical treatment of CFC, effect is obviously, especially fit to older age of CFC or take great efforts to move bowels of CFC patient Can successfully improve the primary symptoms of the patients and induce little side effect. Prescrption which composed of Cistanche salsa 25g, Astragalus membranaceus 20g, Paeonia lactiflora Pall 15gand Pofygonum multiflorum Thunb 1 Og can exert the best eflfect.