The Applied Research Of Uniform Design In Qingyitang Prescription Ratio

Objective:By the mathematical model of the uniform design method to exploratory study the prescription Ratio of Qingyitang, in order to find the new prescription ration of Qingyitang, which could affect the serum amylase value change in the incidence of acute pancreation in the same time compared with the formulation effects,repreed in the literature;Method:120healthy SD rats, Male and female, arranged according to the weight by a random number table to divid into20groups (N=6), number1to16group belong to the different formulations qingyitang treatment group,number17to20group belong to experimental validation group.Making model by injecting5%sodium taurocholate0.2ml/100g to retrograde cholangiopan creatography,close the abdomen while the whole pancreas become reddening and diffuse bleeding point,after making model,gavaging1.5ml Qingyitang in1h,10h,20h administration;The postoperative normal give food and water,24hour later,take sterile syringe portal vein blood4ml,standing,centrifugationand supernatant1.2ml,sending shanxi Medical physiology laboratory to test,by automatic biochemical to analize serum amylase value.Results:(1) To simulate nonlinear regression equation is as follows by using SAS9.2:Y=111.15+17.62x1x2-0.43x3x5+2.49x3x6-2.72x2x7+0.0076x4x5; by using DPS software to optimize the simulation regression equation, The ratio of the respective components of the resultant was:x1=19.7、x2=14.3、x3=19.7、x4=19.7、x5=11.5、 X6=11.9、x7=10.9, Y=5148.9; Of each component corresponding to the actual amount of crude drug ischaihul6.75g, muxiang12.16g, shengdahuang16.75g, baishao16.75g, huangqin9.78g, huanglian10.12g, mangxiao9.27g, Amylase value theoretically5148.9U/L;(2)Ratio of experiment to find a prescription arrangements experiments, Configure the appropriate Qingyitang, Modeling gavage and blood the same way as before, The measured change amylases value5100±496U/L, With the theoretical value of Y=5148.9U/L close,(3) Used by our department the Qingyitang prescription arrange experiments,,, chaihu、huangqin、huanglian、 muxiang、mangxiang all are9g, dahuang15g, The experimental results are shown amylase value of4325±398U/L,(4) Tianjin Nankai Hospital improved prescription (chaihu15g、huangqin10g、huhuanglian10g、baishao15g、muxiang10g、yuanhu10g、dahuang15g、mangxiao10g), The experimental results are shown amylase amylase value of3821.5±370U/L;(5) Use of a relatively large number of selected literature①No. prescription chaihu15g、huangqin10g、 dahuang15g、huanglian12g、yanhusuo12g、muxiang12g、baishao12g, mangxiao10g、houpu9g, The results show its amylase change value of3800±300U/L.(6) Compared with the other three groups recipe, The new prescription Qingyitang obtained by uniform design Amylase changes affect rats with acute pancreatitis is superior to other formulations.Conclusion:(1) Uniform design method can be used for the components of Chinese medicine compound ratio study group ratio optimization and development of new drugs to its unique value;(2) The new prescriptions obtained through the experiment Qingyitang better than the results reported in the literature.