The Effect Of Huang Jin Dan On Blood-Lipid And Lipid Peroxidation Of Experimental Hyperlipemia In Rats

Objectives: To study the effect of huang jin dan on blood-lipid and lipid peroxidation of experimental hyperlipemia in rats.Methods: In this study , animal model of hyperlipidemia in rats has been established by feeding food riched in high cholesterol. From the first day after the animol model has been established,the rats were continuously given by gavage to huang jin dan by low-dose 6.75g/kg.d , middle-dose 20.25g/kg.d, high- dose 40.5g/kg.d for forty days, blood serum total cholesterol (TC),triglyceride (TG) , low density lipoprotein-cholesterol(LDL-C),high density lipoprotein-cholesterol(HDL-C), and high density second subgroup of lipoprotein-cholesterol(HDL2-C) determination in rats on full-automatic biochemistry analyzer with enzymatic reaction; apoproteinA-l(apo A-l) , apoproteinB(apoB) and lipoprotein-cholesterol(a) [Lp ( a ) ] concentration determination on full-automatic biochemistry analyzer with immune turbidimetry; serum malodialdehyde(MDA) concentration determination with thiobarbituric acid method ; serum superoxide dismutase(SOD) activity determination with xanthine oxidase method ; oxidative low density lipoprotein (oxLDL) determination with enzyme-linked immunosorbent assay(ELISA) double antibody with filling method.Results: All three dose of huang jin dan has obviously reduced the concents of blood TC , TG, LDL-C,VLDL-C,apoB, Lp(a), oxLDL,MDA and the ratio of TC/HDL-C of hyperlipidemia in rats, it has obviously raisen theconcents of blood HDL-C, HDL2-C, apoA-I and the activity of SOD , LCAT ; the middle dose group is the most advantage.Cunclusions: huang jin dan has the effect on regulating blood-lipid metabolic disorder as well as preventing lipid peroxidation, it shows huang jin dan has an applied value on preventing and treating the happening and developing of atherosclerosis and atherosclerotic diseases.