| The gastric carcinoma is the most common malignant tumor in the alimentary truct, and its total 5-year-survival rate is under 30 percent. Its clinical stage and prognosis are mainly concerned with the tumor's infiltrated depth and differential degree, the lymph node metastasis and peritoneal metastasis are important factors in evaluating tumor's infiltration and prognosis, and PTNM stage can reflect the gastric carcinoma's clinical biological behaviors better. Peritoneal metastasis is a frequent type of recurrence after surgery and the reason for the poor prognosis of gastric carcinoma.For Traditional Chinese Medicine theory the spleen asthenia syndrome has many pathological features of Chinese Medicine, such as vague, chronic, asthenial, gradually overall changed characteristic in normal body and it can reflect the illness essence more effectively than that of the disease itself.The modern medicial study and clinical research indicate that thespleen asthenia syndrome plays an important role in the gastric carcinoma's formation and progress. The advanced gastric carcinoma is often accompanied by the spleen asthenia syndrome.Our research indicated that the advance gastric carcinoma, particularly accompanied by the spleen asthenia syndrome, had poor prognosis and the treatment effect was bad. The study on treatment for peritoneal metastasis of human gastric cancer with the spleen asthenia syndrome combinated by TCM and western medicine has many problems which ought to be resolved. There were no study on the establishment of model for transplantable peritoneal metastasis of human gastric cancer in nude mice with the spleen asthenia syndrome and the effect of L-OHP, Shenqi Fuzheng Injection, CIK cells on it. There were few reports about human B7-1(CD80), MHC expression on the surface of gastric cancer cells. And there were few reports about relationship between the spleen asthenia syndrome of TCM and the biological behavior in patients with gastric carcinoma.In order to solve these problems, this research was designed to establish a model for transplantable peritoneal metastasis of human gastric cancer in nude mice with the spleen asthenia syndrome and to explore the feasibility of using this model to study the anti-gastric cancer effect of combination of TCM and western medicine. The effect of L-OHP, Shenqi Fuzheng Injection, CIK cells on it was investigated. Furthermore, the effects to biological characteristics and hB7-1, the major histocompatibilitycomples class I, II (MHC I, II) antigen expression on the surface of human gastric carcinoma OCUM-2MD3 cells infected by the recombinant adenovirus(rAd) on the basis of generation of replication-deficient hB7-1 recombinant adenovirus was studied. At the same time, the biological behavior in patients of human gastric cancer with the spleen asthenia syndrome and the effect of Shenqi Fuzheng Injection on their immunological function was investigated too. So the method and mechanism of the treatment for peritoneal metastasis of human gastric cancer with the spleen asthenia syndrome combinated by TCM and western medicine and the theory for its clinical utilization were explored.Parti The Establishment and Biologic Characteristic of Transplantable Peritoneal Metastasis of Human Gastric Cancer in Nude Mice with the Spleen Asthenia SyndromeObjective : To establish a model for transplantable peritoneal metastasis of human gastric cancer in nude mice with the spleen asthenia syndrome. To explore the feasibility of using this model to study the anti-gastric cancer effect of combination of TCM and western medicine.Methods: The Balb/c nu/nu naked mice were divided into 3 groups, control, the spleen asthenia syndrome group (SAS group) and the spleen tonic group (ST group). The nude mice of SAS group and ST group werefeed the Rhubarb and mirabilite Mixture on days to establish the spleen asthenia syndrome model. The ST group were feed Shenqi Fuzheng Injection 3 days and the others were feed cold boiled water. All of the mice were implanted a...
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