| Objective:Allogeneic ( C57BL/6j , H-2Kb, BALB/c , H-2Kd ) and homogenous (BALB/c, H-2Kd) mouse models were employed. Observing homing and survival status of transp- lanted cells in mice after bone marrow transplantation with different ways, and relation that homing cells and expression of chemoattractant SDF-1. To investigate the homing law in time of transplanted cells, the affect to homing and survival rate after bone marrow transplantation of different number cells, the role of chemoattractant SDF-1 in hematopoi- etic cells homing. There are three segments in this study.1. The homing law in time of transplanted cells after bone marrow transplantation(DObservetion of homing effective at different endpoint after different number of allogeneic bone marrow cells transplantation by a single injectionExperimental design:Recipient was BALB/c mouse dividing into 4 groups randomly and each was 24 pieces. After 4 hours of total body lethally irradiation of 60Co-Y ray, 5×105, 1×106, 5×106, 1×10 7 different bumber of cells of donor bone marrow cell (C57BL/6j) was transplanted. 6 pieces mouse were to put to death each group respectively at 24 > 48 > 72 > 96 hour after transplantation, detective for (Dkaryote counting of PBC, BMC, SpC, (2)positive cells of donor. Homing status was observed continuously.Results: The positive cells in peripheral blood, bone marrow and spleen detected by flow cytometer was the highest in third day after different number cells transplanted by single injection(P<0.01) .Average homing cells in bone marrow of transplanted groups from first day to fourth day has significant difference(P<0.05) . Similar to above, the homing efficiency from first to fourth day has also a significant difference (P<0.05) . But homingtendency was different in spleen from bone marrow.(2)0bservetion of homing effective at different endpoint after different times transplantation of mix bone marrow cellsExperimental design: Recipient was BALB/c mouse dividing as 1 time, 2times, Stimes, 4times 4 groups randomly and each was 8 pieces. Donor mouse were C57BL/6j and BALB/c mouse, transplanted number of cells per fractionation was 2. 5X lOYpiece, and transplanted sequence as follow:endpoint 4h 24h 48h 72h1 time C57BL/6j2 times BALB/c C57BL/6j3 times BALB/c BALB/c C57BL/6j4 times BALB/c BALB/c BALB/c C57BL/6j endpoint: After total body lethally irradiation of 60Co-y rayEach group mouse was put to death respectively in 24 hours transplanted C57/bj bone marrow cells, detective for (l)karyote counting of PBC> BMC> SpC, (2)positive cells of donor, aim is to compare homing status of per franctionation.Results:Higher positive cells was observed every detection after mix allogeneic and homogenous bone marrow cells transplant ion, the positive cells detected by flow cytometer in bone marrow was significant higher than it in peripheral blood and spleen, and the highest ratio of positive cell in bone marrow was the group that 2 injections (P<0. 01) .The highest positive cell ratio in spleen was group by single injection. The highest homing number of positive cells and homing efficiency in bone marrow was 2 injections group, and homing number of positive cells and homing efficiency in spleen was seen in single injection group (P<0.05) .(3)Observation of homing law at different endpoint after allogeneic bone marrow cells by different times transplantationExperimental design: Recipient was BALB/c mouse dividing as1 time, 2times, Stimes, 4times 4 groups randomly and each was 24 pieces. Donor mouse were C57BL/6j mouse and transplanted number of cells per fractionation was 2. 5 X lOVpiece, and transp- lanted sequence as follow (Cg7BL/6j is transplanted sequence, 6pieces is detective, endpoint is time after total body lethally irradiation of 60Co- y ray) :endpoint Itime 2times Stimes 4times4h C57BL/6j C57BL/6j C57BL/6j C57BL/6j24h 6 piece... |
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