| Objective To establish the rabbit model of corneal neovascularization with the aim of observing the changing course of CoNV and then selecting the timing of laser intervention. Methods Bilateral corneas of ten white New Zealand rabbits were sutured and two rabbits as control. The suture was removed after ten days. The observation of anterior part of eyeball using slit lamp and fluorescein angiography (FA) were conducted immediately, 3 days, 2 weeks, 1 month, 2 months after removing the suture. Both eyes of random two rabbits were excised for histological examination at each observation point. Results Neovascularization had reached the end of suture 10 days after suturing. Obvious edema of cornea at the site of suturing and dilation, congestion of neovascularization were observed when suture was removed. The examination of FA showed obvious exudation of CoNV. Corneal edema disappeared and the sum of CoNV decreased gradually following observation time. Examination of histology showed CoNV was located in anterior and middle stroma of cornea. There were obvious inflammatory cell infiltration 3 days after suture removing, however tissue edema and inflammatory cell infiltration was lighten 2 weeks after suture removing. Conclusion Suturing could induce formation of CoNV. This type of CoNV belongs to superficial or middle CoNV and has the tendency of auto recession. 3 days and 2 weeks after suture removing could be regarded as active stage and static stage of CoNV respectively to perform laser intervention.
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