| Objective To determine the relationship of the polymorphism of coagulation factors Ⅶgene and Angiotensin-converting enzyme gene with the heart blood stasis syndrome (HBSS) in coronary heart disease.Method 1. Investigation of literature: The literature of today and old was studied systematically, to know the character of distribution and research progression of HBSS of coronary heart disease(CHD).2. The epidemiological study of heredity tendency of HBSS: 54 patient with HBSS and 54 healthy people were investigated by genealogy analysis method, to detect the incidence rate and heritability of the first grade relations, the relative risk and 95% confidence interval of episode of the children whose parents were in three kinds of match types.3. The different patients with different Chinese medicine syndromes of CHD and healthy people were detected by polymerase chain reaction (PCR) for their angiotensin-converting enzyme (ACE) genetypes and allele frequency. The factors of vessel endothelium such as angiotensin II (AgII), nitrogen monoxide (NO) and endothelins (ET) were tested. All these information were analysised to determine the relationship of the insertion/detection polymorphism of ACE gene with HBSS of coronary heart disease.4. By polymerase chain reaction - restriction fragment length polymorphism (PCR- RFLP), the patients with different Chinese medicine syndromes in CHD and healthy people were detected for their coagulation factors VII (FVII) gene and allele frequency. At the same time, the activity of FVII, antithrombin III (ATIII), GMP-140, activator of plasminogen (tPA), plasminogen activator inhibitor(PAI)-1 were tested.These factors which reflect the functions of coagulation and fibrinolytic system were analysised to determine the relationship of coagulation factors gene with HBSS in CHD. Results 1. The literature indicated: That HBSS is the most frequent in the TCM syndromes in CHD. There are the function disorders of coagulation, anti-coagulation , fibrinolysis and gene expressiving abnormily in BSHVS of CHD, CHD occurring is related to some predisposing genes.2. 54 patients with HBSS and 54 healthy people were investigated by genealogy analysis method. The results showed that the incident rate of the first grade relations of patient with HBSS was 7.62%, the rate of the control health group was 0.95%. The differences between two groups were significant. The heritability of HBSS was 67.4±6.89%; the group whose parents are all healthy was as the control group; the RR of group whose one of parents were with HBSS was 3.46, the RR of group whose parents are all with HBSS was 5.84. there were causality in RR of HBSS of parents and RR of children. The HBSS is a polygene syndrome that has the tendency of heredity.3. By researching the relationship of HBSS of CHD with polymorphism of ACE gene and the vessel endoctheial function, the frequence of DD genetype and allele of HBSS group was greater than none-HBSS group and healthy group (p<0.01). There were significant statistic differences among odds rate (OR) which HBSS group compared with none-HBSS group, healthy group and all observation objects. the levels of AgII, ET/NO of HBSS group are significantly higher than none-HBSS group and healthy group (p<0.05 or 0.01). The DD genetype group had a highest level. These showed that the vessel endothelial functions of HBSS group with genetype DD were damaged deeply. 4. By the research of the relationship of HBSS and FⅦgene type, allele frequency and the function of coagulation and fibrinolytic system, we found that the frequence of M1M1 gene type and allele of HBSS group was greater than none-HBSS group and healthy group, but difference was not significant (p>0.05). Because the number of samples is maybe small , we did not find that there were association of HBSS of CHD with the FⅦ gene Msp1 polymorphism. By testingthe activity of coagulation factors VII (FVIIc), antithrombin III (ATIII), GMP-140, activator of plasminogen (tPA), plasminogen activator inhi...
|
PDF Full Text Request